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Multicolor Staining Guide

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With wide varieties of antibodies, fluorophores, and instruments available for multicolor flow cytometry, optimizing your multicolor experiments can be confusing and frustrating. Use our Multicolor Staining Guide to assist you in developing and optimizing your flow cytometry experiments. Master the five aspects of the Staining Guide and you will be well on your way to legendary discovery. When you are ready to find antibodies, go to the Multicolor Panel Selector.

Fluorophore Brightness

It is important to understand the properties and capabilities of each fluorophore that you may use for multicolor flow cytometry. One such important property is the relative brightness of the fluorophore when conjugated to antibodies. We provide these value in terms of Relative Brightness Index. Brightness Index values are not absolute and can vary depending on the antibody, the antigen, the instrument and its configuration, the staining protocol, the cell type, and other factors. The Brightness Index is provided as a general guide to help in multicolor panel construction, with 1 being the dimmest and 5 being the brightest. The tables below also provide emission spectra and important comments on the use of each fluorophore. Explore fluorophores by laser excitation with the tabs below. Also use our Fluorescence Spectra Analyzer or Aurora Spectra Analyzer to compare and analyze excitation and emission data. Learn more about BioLegend’s Tandem Dyes.

Fluorophore Brightness Index (1=dim, 5=bright) Exmax Emmax Comments
Spark PLUS UV395™ 4 355 nm 385 nm Spark PLUS UV395™ is a bright UV-excited dye and the first in a family of brighter Spark Dye options. This dye is useful for conventional and spectral cytometers with excellent stability when exposed to heat or fixative.
Spark UV™ 387 2 351 nm 387 nm Spark UV™ 387 can easily be used alongside BUV496™ and Brilliant Violet™ dyes in a multicolor panel. It has excellent stability when exposed to heat, light, and fixatives.

 

Fluorophore Brightness Index (1=dim, 5=bright) Exmax Emmax Comments
Brilliant Violet 421™ 5 405 nm 421 nm Brilliant Violet 421™ is a novel organic fluorescent polymer capable of extremely bright signal and high photostability. It is an excellent choice for flow cytometry and microscopy. It is not recommended for use with Pacific Blue™.
Spark Violet™ 423 3 400 nm 415, 423 nm Spark Violet™ 423 can be unmixed from Brilliant Violet 421™ and Pacific Blue™ on spectral cytometers. Spark Violet™ 423 has peak emission at 415 and 423 nm.
Pacific Blue™ 1 401 nm 455 nm Pacific Blue™ is a hydroxycoumarin-based fluorophore, generally considered to be dimmer than most other fluorophores. It is recommended for highly expressed antigens. Brilliant Violet 421™ is a brighter alternative for weakly expressed antigens. 
Spark Violet™ 500 1 393 nm 500 nm Spark Violet™ 500 is a violet laser option that can replace BD Horizon™ V500 (V500-C) or AmCyan. This dye can be cocktailed without inter-dye interaction and is easily unmixed from neighboring dyes on spectral instruments.
Brilliant Violet 510™ 2 405 nm 510 nm Brilliant Violet 510™ is a novel polymer with excellent signal-to-noise, excited by the violet laser. It can provide dramatic improvements over existing fluorophores emitting in this range such as Pacific Orange™ and Horizon™ V500.
Spark Violet™ 538 1 399 nm 538 nm Spark Violet™ 538 is a brighter alternative to Pacific Orange™ and can be successfully unmixed from neighboring dyes such as Brilliant Violet 510™, Brilliant Violet 570™, and Brilliant Violet 605™. 
Brilliant Violet 570™ 2 405 nm 570 nm Brilliant Violet 570™ is a novel organic fluorescent polymer. It provides a much brighter alternative to Pacific Orange™ for multicolor flow on the violet laser and is a better alternative to nanocrystals for intracellular flow cytometry.
Brilliant Violet 605™ 3 405 nm 603 nm Brilliant Violet 605™ is a novel organic fluorescent polymer. It provides a much brighter alternative to eFluor® 605 for multicolor flow on the violet laser and is a better alternative to nanocrystals for intracellular flow cytometry.
Brilliant Violet 650™ 4 405 nm 646 nm Brilliant Violet 650™ is a novel organic fluorescent polymer. It provides a much brighter alternative to eFluor® 650 for multicolor flow on the violet laser and is a better alternative to nanocrystals for intracellular flow cytometry.
Brilliant Violet 711™ 5 405 nm 711 nm Brilliant Violet 711™ is a novel molecule based on the Brilliant Violet 421™ polymer core. It provides a much brighter alternative to eFluor® 700NC for multicolor flow on the violet laser and is a better alternative to nanocrystals for intracellular flow cytometry.
Brilliant Violet 750™ 3 405 nm 750 nm Brilliant Violet 750™ is a novel molecule based on the Brilliant Violet 421™ polymer core. It provides further options for the violet laser, particularly for those with either a spectral detection cytometer or a cytometer with a decagon configuration for the violet laser. Alternatively, it can be used in place of BV785™ on a standard violet laser octagon configuration.
Brilliant Violet 785™ 4 405 nm 785 nm Brilliant Violet 785™ is a novel molecule based on the Brilliant Violet 421™ polymer core. It provides further options for the violet laser and is a better alternative to nanocrystals for intracellular flow cytometry.

 

Fluorophore Brightness Index (1=dim, 5=bright) Exmax Emmax Comments
Spark Blue™ 515 2 506 nm 515 nm Spark Blue™ 515 is a blue laser option that emits at 515 nm. As its peak emission is approximately 10 nm lower than dyes like FITC and Alexa Fluor® 488, it can be unmixed from them on spectral instruments.
Alexa Fluor® 488 3 495 nm 519 nm Alexa Fluor® 488 is a fluorescent molecule with exceptional photostability. For flow cytometry it has very similar brightness and emission properties compared to FITC, but is advantageous for microscopy applications. 
KIRAVIA Blue 520™ 4 488 nm 520 nm KIRAVIA Blue 520™ is bright, on par with BD Horizon™ BB515 and, on average, is twice as bright as a FITC conjugate. In a multicolor panel, it is excited by the 488 nm blue laser and spectrally identical to FITC, only exhibiting significant spreading into Spark Blue™ 550.
FITC 3 494 nm 520 nm FITC (fluorescein isothiocyanate) is a small synthetic organic fluorophore with moderate brightness, suitable for flow and microscopy applications. It is sensitive to pH changes. It cannot be used with Alexa Fluor® 488.
PerCP 1 482 nm 678 nm PerCP is a naturally-derived carotenoid pigment found in photosynthetic dinoflagellates. It is not recommended for high powered lasers >25 mW due to its sensitivity to photobleaching. PerCP has overlapping emission with PE/Cyanine5, so these should not be used on the same laser.
PerCP/Cyanine5.5 2 482 nm 690 nm PerCP/Cyanine5.5 is tandem conjugate of PerCP and the cyanine dye, Cyanine5.5. Unlike PerCP, it is quite photostable and can be used with high powered lasers. Of the all tandems, it is the most photostable and resistant to degradation by fixation. PerCP and PerCP/Cyanine5.5 have significant overlap and are not recommended for use together.
Spark PLUS B550™ 4 516 nm 540 nm Spark PLUS B550™ is formulated for brighter signal and superior stability, maximizing signal from the blue laser with minimal yellow/green cross-excitation and spillover.
Spark Blue™ 550 1 516 nm 540 nm Spark Blue™ 550 is designed to be used in high level (>22 color) flow cytometry assays on a spectral unmixing cytometer. It is excited off of the 488 nm blue laser and emits between the peak emission of FITC and PE. Emission off the 405 nm violet laser adds to the accuracy with which the fluorophore can be unmixed. It can be used as an alternative to Alexa Fluor® 532.
Spark Blue™ 574 1 506 nm 574 nm Spark Blue™ 574 is ideal for 5-laser spectral unmixing cytometers as it is mainly excited by the 488 nm blue laser. With minimal excitation from the 561 yellow/green laser, this allows it to be unmixed from PE. Spark Blue™ 574 has a peak emission of 574 nm.
PE 5 565 nm 578 nm R-Phycoerythrin (PE) is a pigment derived from red algae. It is a large 240 kD protein that contains 23 phycoerthrobilin chromophores per molecule. It is extremely bright for flow cytometry, but poor photostability makes it unsuitable for microscopy. PE and its tandem are also sensitive to denaturation by fixation. Because of its wide excitation range, it can be excited by the 488, 532, and 561 nm lasers.
PE/Dazzle™ 594 5 565 nm 610 nm PE/Dazzle™ 594 is a tandem conjugate of PE and BioLegend’s proprietary fluorophore DZL594. Similar to PE, it is extremely bright for flow cytometry. It is spectrally similar to PE-Texas Red®, BD Horizon™ PE-CF594, and PE-eFluor® 610.
PE/Fire™ 640 4 565 nm 639 nm PE/Fire™ 640 has a distinct emission peak between the peaks of PE/Dazzle™ 594 and PE/Cyanine5 which allows it to be unmixed from highly overlapping fluorophores in spectral cytometry applications. Because it has a bright signal, it is ideal for labeling an antigen that is expressed on a small subset of cells.
PE/Cyanine5 5 565 nm 667 nm PE/Cyanine5 is a tandem conjugate of PE and the cyanine dye, Cyanine5. Similar to PE, it is bright for flow cytometry, but is sensitive to photobleaching and not recommended for microscopy. Cyanine5 has been known to non-specifically bind to Fc receptors. On some instruments, it is not recommended for use with APC, due to their overlapping emissions.
PE/Fire 700™ 5 565 nm 695 nm PE/Fire™ 700 is an intensely bright fluorophore that is best suited for detecting antigens that are lowly or discreetly expressed. It is best suited for spectral cytometers but can also be used on conventional cytometers that are able to detect PE/Cyanine5.5. While it is a very bright fluor, it also exhibits significant spillover into all other PerCP and PE tandems, as well as Spark NIR™ 685 and Alexa Fluor® 700.
PE/Fire 744™ 5 565 nm 744 nm PE/Fire™ 744 is excited by the blue and yellow/green lasers and has a max emission of 744 nm. It can be unmixed from PerCP/Fire™ 780 and PerCP/Fire™ 806 on spectral instruments.
PE/Cyanine7 4 565 nm 774 nm PE/Cyanine7 is a tandem conjugate of PE and the cyanine dye, Cyanine7. Similar to PE, it is bright for flow cytometry, but is sensitive to photobleaching and not recommended for microscopy. This tandem is particularly sensitive to light exposure and prolonged fixation.
PerCP/Fire™ 780 4 488 nm 774 nm PerCP/Fire™ 780 is a blue laser option that emits deep in the far red at 774 nm. It can be used with PE/Cyanine7 on instruments with blue and yellow/green lasers. It can be unmixed from PerCP/Fire™ 806 on spectral instruments.
PerCP/Fire™ 806 4 488 nm 806 nm PerCP/Fire™ 806 is a blue laser option that emits deep in the far red at 806 nm. It can be unmixed from dyes like PE/Fire™ 810 and PE/Cyanine7 on spectral instruments.
PE/Fire™ 810 4 565 nm 806 nm PE/Fire™ 810 expands the range of spectral detection further than any of our previous fluorophores, emitting even beyond the range of PE/Cyanine7. PE/Fire™ 810 is excited by the blue and yellow/green lasers and can utilized for nearly any antigen since it has little spectral overlap with other fluorophores.
Fluorophore Brightness Index (1=dim, 5=bright) Exmax Emmax Comments
PE 5 565 nm 578 nm R-Phycoerythrin (PE) is a pigment derived from red algae. It is a large 240 kD protein that contains 23 phycoerthrobilin chromophores per molecule. It is extremely bright for flow cytometry, but poor photostability makes it unsuitable for microscopy. PE and its tandem are also sensitive to denaturation by fixation. Because of its wide excitation range, it can be excited by the 488, 532, and 561 nm lasers.
PE/Dazzle™ 594 5 565 nm 610 nm PE/Dazzle™ 594 is a tandem conjugate of PE and BioLegend’s proprietary fluorophore DZL594. Similar to PE, it is extremely bright for flow cytometry. It is spectrally similar to PE-Texas Red®, BD Horizon™ PE-CF594, and PE-eFluor® 610.
PE/Fire™ 640 4 565 nm 639 nm PE/Fire™ 640 has a distinct emission peak between the peaks of PE/Dazzle™ 594 and PE/Cyanine5 which allows it to be unmixed from highly overlapping fluorophores in spectral cytometry applications. Because it has a bright signal, it is ideal for labeling an antigen that is expressed on a small subset of cells.
PE/Cyanine5 5 565 nm 667 nm PE/Cyanine5 is a tandem conjugate of PE and the cyanine dye, Cyanine5. Similar to PE, it is bright for flow cytometry, but is sensitive to photobleaching and not recommended for microscopy. Cyanine5 has been known to non-specifically bind to Fc receptors. On some instruments, it is not recommended for use with APC, due to their overlapping emissions.
PE/Fire 700™ 5 565 nm 695 nm PE/Fire™ 700 is an intensely bright fluorophore that is best suited for detecting antigens that are lowly or discreetly expressed. It is best suited for spectral cytometers but can also be used on conventional cytometers that are able to detect PE/Cyanine5.5. While it is a very bright fluor, it also exhibits significant spillover into all other PerCP and PE tandems, as well as Spark NIR™ 685 and Alexa Fluor® 700.
PE/Fire 744™ 5 565 nm 744 nm PE/Fire™ 744 is excited by the blue and yellow/green lasers and has a max emission of 744 nm. It can be unmixed from PerCP/Fire™ 780 and PerCP/Fire™ 806 on spectral instruments.
Spark YG™ 581 2 565 nm 581 nm Spark YG™ 581 is specifically excited by the 561 nm yellow/green laser, with minimal cross-excitation from the 488 nm blue laser. On a spectral cytometer, it can be successfully unmixed from PE, making it ideal for larger panels.
Spark YG™ 593 3 574 nm 593 nm Spark YG™ 593 is specifically excited by the 561 nm yellow/green laser, with minimal cross-excitation from the 488 nm blue laser. It can be successfully unmixed from PE and emits between PE and PE/Dazzle™ 594.
PE/Cyanine7 4 565 nm 774 nm PE/Cyanine7 is a tandem conjugate of PE and the cyanine dye, Cyanine7. Similar to PE, it is bright for flow cytometry, but is sensitive to photobleaching and not recommended for microscopy. This tandem is particularly sensitive to light exposure and prolonged fixation.
PE/Fire™ 810 4 565 nm 806 nm PE/Fire™ 810 expands the range of spectral detection further than any of our previous fluorophores, emitting even beyond the range of PE/Cyanine7. PE/Fire™ 810 is excited by the blue and yellow/green lasers and can utilized for nearly any antigen since it has little spectral overlap with other fluorophores.
Fluorophore Brightness Index (1=dim, 5=bright) Exmax Emmax Comments
APC 4 650 nm 660 nm Allophycocyanin (APC) is a 105 kD molecule derived from blue-green algae that has 6 phycocyanobilin chromophores per molecule. It is bright and particularly useful for flow cytometry but not microscopy. It should not be used together with Alexa Fluor® 647 due to their overlapping emissions.
Alexa Fluor® 647 4 650 nm 668 nm Alexa Fluor® 647 is spectrally equivalent to APC, but has much better photostability, making it ideal for microscopy applications. While generally not as bright as APC, it is also a good choice for flow cytometry.
Alexa Fluor® 660 1 663 nm 690 nm Alexa Fluor® 660 is a small, photostable organic dye that emits in the red side of the light spectrum. It is best excited by the 633 or 640 nm laser lines. Due to its excitation peak being similar to other dyes, such as Alexa Fluor® 700, careful consideration must be taken when incorporating it into the design of a multicolor flow cytometry panel.
Spark NIR™ 685 3 660 nm 685 nm Spark NIR™ 685 is designed to be used in high level (>22 color) flow cytometry assays on a spectral unmixing cytometer. It emits between the peak emission of Alexa Fluor® 647 and Alexa Fluor® 700. It can be used in the same panel as Alexa Fluor® 647 but ideally, they should not be used to detect two co-expressed markers.
Spark Red™ 718 4 697 nm 711 nm Spark Red™ 718 is a brighter alternative to Alexa Fluor® 700, adding to the fluorophore options for the red laser that can detect antigens with lower expression.
Alexa Fluor® 700 2 696 nm 719 nm Althought it is a dim molecule, Alexa Fluor® 700, expands the choices for the red laser, fitting nicely between APC and APC/Cyanine7.
APC/Cyanine7 2 650 nm 774 nm APC/Cyanine7 is a tandem of APC and the cyanin dye, Cyanine7. It is particularly sensitive to light and fixation and should be handled with care to avoid light exposure.
APC/Fire™ 750 2 650 nm 774 nm APC/Fire™ 750 is a new replacement for APC/Cyanine7 with improved temperature and photostability. APC/Fire™ 750 also has lower compensation requirements than APC/Cyanine7 conjugates while maintaining an equal level of brightness. Additionally, APC/Fire™ 750 has minimal non-specific binding to monocytes, as has been observed with APC/Cyanine7. Lastly, APC/Fire™ 750 conjugates are consistently brighter than APC-H7 in all conjugates tested.
APC/Fire™ 810 3 650 nm 807 nm APC/Fire™ 810 expands the range of spectral detection further than any of our previous fluorophores. In a large multicolor panel, it can be assigned to nearly any antigen because it exhibits minimal spillover into other channels and emits far outside of the autofluorescence range. APC/Fire™ 810 is excited by the red laser and can be easily combined with other red laser fluorophores when using a spectral cytometer.

View our complete Brightness Index

 

Our technical service team is available to help with any questions you may have on multicolor flow cytometry.

Technical Service
Phone Toll-Free (US & Canada)1-877-273-3103
Phone (International): 1-858-768-5801 
Email: Click Here

For a complete listing of the trademarks and patents referenced on this page, click here.

PE/Fire™ 780 is an equivalent fluor to PE/Cyanine7. It is restricted to ASR use only and is only available to US customers.

Your Instrument

Before undertaking any flow cytometry experiments, it is important to know the capabilities of your instrument. You will need to define exactly which fluorophores you can or cannot use on your available flow cytometers. To find more information, use our Instrument Guide/Fluorophore Selector Tool linked below, check your cytometer manual, or check with your local flow core facility. Click on the image below to use the Instrument Guide/Fluorophore Selector Tool.

 

Our technical service team is available to help with any questions you may have on multicolor flow cytometry.

Technical Service
Phone Toll-Free (US & Canada)1-877-273-3103
Phone (International): 1-858-768-5801 
Email: Click Here

For a complete listing of the trademarks and patents referenced on this page, click here.

PE/Fire™ 780 is an equivalent fluor to PE/Cyanine7. It is restricted to ASR use only and is only available to US customers.

Our technical service team is available to help with any questions you may have on multicolor flow cytometry.

Technical Service
Phone Toll-Free (US & Canada)1-877-273-3103
Phone (International): 1-858-768-5801 
Email: Click Here

For a complete listing of the trademarks and patents referenced on this page, click here.

PE/Fire™ 780 is an equivalent fluor to PE/Cyanine7. It is restricted to ASR use only and is only available to US customers.
 

Expression of Common Markers

Knowing the relative expression of antigen markers can help you optimize your fluorophore selections in multicolor flow cytometry. In general, select bright fluorophores for your weakly expressed antigens and select dimmer fluorophores for highly expressed antigens. This strategy provides more manageable compensation for your samples and gives you the most accurate data for weakly expressed antigens.

View Expression of Common Surface Molecules on Blood Cells

Our technical service team is available to help with any questions you may have on multicolor flow cytometry.

Technical Service
Phone Toll-Free (US & Canada)1-877-273-3103
Phone (International): 1-858-768-5801 
Email: Click Here

For a complete listing of the trademarks and patents referenced on this page, click here.

PE/Fire™ 780 is an equivalent fluor to PE/Cyanine7. It is restricted to ASR use only and is only available to US customers.

The Rules

There are a few basic rules to follow when setting up a multicolor panel. Follow these rules to organize and optimize your results.

  1. Choose the brightest fluorophore for your least expressed protein and the dimmest fluorophore for your most highly expressed protein. To help you, we have compiled a chart to indicate the expression of common surface molecules on blood cells. Generally, molecules like CD45, CD3, CD4, and CD8 are quite highly expressed on their target cell types and can be used quite successfully with dim fluorophores.
  2. Choose fluorophores with emissions having the least spectral overlap. For example, although Brilliant Violet 421™ and Pacific Blue™ do not have exact the same emission spectra, they did have significant overlap, so you should generally avoid using these together. Use our Spectra Analyzer to view and compare excitation and emission spectra, view laser lines, and add custom bandpass filters.
  3. Use tandems (PE/Cyanine5, PE/Cyanine7, APC/Cyanine7) with caution, as they are more susceptible to degradation by light exposure or fixation. They are essential to large multicolor panels, so just use them with care to prevent light exposure and use appropriate fixation buffers and protocols.
  4. Avoid acidic buffer conditions with FITC labeled samples because FITC is sensitive to low pH.
  5. Avoid exposing stained samples to bright light as most fluorophores are susceptible to photobleaching, causing them to lose fluorescence. This is particularly true for tandems dyes.
  6. Avoid incubating cells in fixative for extended periods of time, as this may affect fluorescence, particularly of tandems dyes.

Our technical service team is available to help with any questions you may have on multicolor flow cytometry.

Technical Service
Phone Toll-Free (US & Canada)1-877-273-3103
Phone (International): 1-858-768-5801 
Email: Click Here

For a complete listing of the trademarks and patents referenced on this page, click here.

PE/Fire™ 780 is an equivalent fluor to PE/Cyanine7. It is restricted to ASR use only and is only available to US customers.

For more useful tools and information, see BioLegend’s complete set of webtools, as well as other external resources provided below.

Fluorescence Spectra Analyzer

 

BioLegend's Fluorescence Spectra Analyzer is useful for the analysis of excitation and emission spectra of commonly used fluorochromes for flow cytometry. You can also create custom bandpass filters, export images, and bookmark your settings. Unlike other fluorescence spectra tools on the internet, this Analyzer does not use Java, which allows it to work well on the iPhone and iPad, viewable from your standard web browser.

Try it now!

 

 Multicolor Panel Selector

 

BioLegend’s Multicolor Panel Selector is a multifaceted tool designed to help you fnd the right products for your multicolor flow cytometry experiments. Featuring:
 

  • Simple interface for panel construction
  • Product previews, including data
  • Emission spectra preview and fluorophore notes
  • Fluorophore brightness index
  • Add panel items directly to your shopping cart
  • Isotype controls


The tool also provides tips and tricks to constructing optimized multicolor panels.

Try it now!

 

Purdue Archive

 

Purdue University Cytometry Laboratories’ Email Archive Search through the email archive for questions and discussions, or ask your own question to the flow cytometry community.


Purdue Cytometry Archives

Try it now!

 

United States Organizations

Albert Einstein College of Medicine

Carver College of Medicine-University of Iowa

Great Lakes International Flow Cytometry association

International Center for Analytical Cytology

Los Alamos National Laboratory

National Flow Cytometry Resource

New England Cytometry User's Group

Purdue University Cytometry Laboratories

Salk Institute

Stanford Shared FACS Facility

University of California San Diego Medical Center

University of Massachusetts Flow Cytometry Core Laboratory

University of Washington Department of Immunology

International Organizations

Literature

 

Books

  1. Darzynkiewicz Z,Roederer M,Tanke H,eds. Cytometry. 4th ed. Boston: Elesevier Academic Press;2004. ISBN:0125641702.
  2. Darzynkiewicz Z.Flow Cytometry. 2nd ed. San Diego,Ca: Academic Press;1994. ISBN:0125641427.
  3. Darzynkiewicz Z,Chrissman HA,Robinson JP,eds. Methods in Cell Biology:Cytometry. 3rd ed. San Diego,CA: Academic Press;200;63 (PT.A).
  4. Givan AL.Flow Cytometry:First Principles. New York,2nd ed. NY:Wiley-Liss;2001. ISBN0471382248.
  5. Grogan WM, Collins JM. Guide to Flow Cytometric Methods. New York,NY:Marcel Dekker;19890. ISBN 0824783301.
  6. Nguyen DT,Diamond LW, Braylan RC. Flow Cytometry in Hematopathology: A Visual Approach to Data Analysis and Interpretation.Totowa,NJ:Human Press;2002. ISBN 1588292126.
  7. Nunez R. Flow cytometry for research scientists:principles and applications. Norwich,UK; Horizon Scientific Press;2001. ISBN 1898486263.
  8. Riley RS. Clinical Applications for Flow Cytometry.New York,NY:Igaku-Shoin;1993. ISBN 0896402002.
  9. Shapiro H.Practical Flow Cytometry. 4th ed. New York,NY: Alan R. Liss; 2003. ISBN 0471411256.
  10. Watson JV. Introduction to Flow Cytometry. New York,NY:Cambridge University Press;1991. ISBN 0521380618.


Periodicals

  1. Cytometry Part B: Clinical Cytometry
  2. Current Protocols in Cytometry
  3. Current Protocols in Immunology
  4. Cytometry Part A
  5. Journal of Immunoassay and Immunochemistry

Our technical service team is available to help with any questions you may have on multicolor flow cytometry.

Technical Service
Phone Toll-Free (US & Canada)1-877-273-3103
Phone (International): 1-858-768-5801 
Email: Click Here

For a complete listing of the trademarks and patents referenced on this page, click here.

PE/Fire™ 780 is an equivalent fluor to PE/Cyanine7. It is restricted to ASR use only and is only available to US customers.

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