10x Genomics with TotalSeq™

Partnerships and Service Providers10x Genomics with TotalSeq™

10x Genomics with TotalSeq™

10x Genomics is a leader in the field of single-cell genomics. Their flagship Chromium platform instruments and reagents for single-cell RNA sequencing enable the simultaneous analysis of gene expression and protein expression for cell type identification to unravel complex biological questions at the single-cell level.

Compatible TotalSeq™ Formats:
TotalSeq™-A, B, and C

Instruments:

10x Chromium X Series

10x Chromium Controller

Assay Compatibility

10x Genomics Assays	Single Cell Gene Expression	Single Cell Immune Profiling	Single Cell Gene Expression Flex
Description	3’ gene expression with options for multiomic analysis	Comprehensive analysis of immune diversity, combining 5’ gene expression with multiomic profiling	Probe based whole transcriptome gene expression, compatible with fresh or fixed samples
Instrument Compatibility	Chromium Controller	Chromium Controller	Chromium X Series
Instrument Compatibility	Chromium X Series	Chromium X Series
Additional Cellular Features	Cellular Protein Detection	Cellular Protein Detection	Cellular Protein Detection
	CRISPR Screening	Antigen Specificity
		BCR/TCR Sequencing
		CRISPR Screening
TotalSeq Format Compatibility	TotalSeq-A*	TotalSeq-C	TotalSeq-B
	TotalSeq-B		TotalSeq-B
	TotalSeq-B		TotalSeq-C
Multiplexing Compatibility	TotalSeq-A Hashtags*	TotalSeq-C Hashtags*	TotalSeq-B Hashtags*
Multiplexing Compatibility	TotalSeq-B Hashtags*	TotalSeq-C Hashtags*	Built-in sample multiplexing

*Use of TotalSeq-A reagents with the 10x Single Cell Gene Expression requires reagent and protocol modifications. 10x Genomics provides limited support for TotalSeq-A, and cell hashing is not officially supported by 10x Genomics. For additional information regarding use of TotalSeq-A or TotalSeq Hashtags, please contact BioLegend Technical Support.

Workflows

Single Cell Gene Expression and Single Cell Immune Profiling

Single Cell Gene Expression Flex

Support and User Guides

10x General Support	Single Cell Gene Expression	Single Cell Immune Profiling	Single Cell Gene Expression Flex
10x Cell Surface Protein Support	Single Cell Gene Expression with Cell Surface Protein Labeling	Single Cell Immune Profiling with Cell Surface Protein	Fixed RNA Profiling with Cell Surface Protein Labeling
10x User Guides	User Guides	User Guides	User Guides
BioLegend TotalSeq Staining Protocols	TotalSeq-A Antibodies and Cell Hashing with 10x Single-Cell 3' Reagent Kit v3.1 (Dual Index) Protocol	TotalSeq-B or -C with 10x Feature Barcoding Technology	TotalSeq-B Cell Surface and Intracellular Cytoplasmic Protein Staining with 10x Chromium Single Cell Gene Expression Flex
BioLegend TotalSeq Staining Protocols	TotalSeq-B or -C with 10x Feature Barcoding Technology	TotalSeq-B or -C with 10x Feature Barcoding Technology

TotalSeq™ Reagents

TotalSeq-A: Designed to work with platforms that rely on poly(dT) oligonucleotides as the mRNA capture method. TotalSeq-A antibodies contain a poly(A) sequence that mimics a natural mRNA. Compatible with 10x Genomics Chromium Single Cell Expression Solution with Cell Surface Protein Labeling. Use of TotalSeq-A reagents with the 10x Single Cell Gene Expression requires reagent and protocol modifications; 10x Genomics provides only limited support for TotalSeq-A. For additional information regarding the use of TotalSeq-A reagents, please contact BioLegend Technical Support.

TotalSeq-B: Capture sequence is compatible with capture sequence 1 found on the Gel Beads used with the 10x Genomics Chromium Single Cell Gene Expression solution and 10x Genomics Single Cell Gene Expression Flex solution with Cell Surface Protein Labeling. Use of TotalSeq B with 10x his is fully supported by 10x Genomics.

TotalSeq-C: Capture sequence is compatible with the TSO capture sequence found on Single Cell 5' Gel Beads used with the 10x Genomics Chromium Single Cell Immune Profiling solution (5') which allows for immune repertoire profiling of T and B cell receptors at a single-cell resolution. TotalSeq-C is also compatible with the 10x Genomics Single Cell Gene Expression Flex solution. The use of TotalSeq-C antibodies is fully supported by 10x Genomics.

We offer single TotalSeq antibody conjugates, pre-titrated optimized cocktails, and hashtags for sample multiplexing in both human and mouse reactivity.

All Products	TotalSeq-A	TotalSeq-B	TotalSeq-C
Cocktails	TotalSeq-A Cocktails	TotalSeq-B Cocktails	TotalSeq-C Cocktails
Hashtags	TotalSeq-A Hashtags	TotalSeq-B Hashtags	TotalSeq-C Hashtags

Explore BioLegend and 10x Genomics Data Sets

BioLegend TotalSeq Universal Cocktail Data Sets

10x Single Cell Gene Expression with Cell Surface Protein Labeling Data Sets

10x Single Cell Immune Profiling with Cell Surface Protein Data Sets

10x Fixed RNA Profiling with Cell Surface Protein Labeling Data Sets

Additional TotalSeq™ Resources

TotalSeq Resources and Learning

Barcode Look-Up - Access our barcode look-up table to view and export barcode information for all of our TotalSeq reagents

10x Genomics with TotalSeq™