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| In response to viral infection, plasmacytoid dendritic cells (pDCs) produce interferons, most notably type I and type II. As the anti-viral immune response is a tightly regulated process, Liu et al. studied how a microRNA can modulate cytokine production in response to TLR stimulation. Expression of microRNA-21 is induced in pDCs following TLR stimulation and deletion of this microRNA results in impaired production of both IFN-α and IFN-γ. Investigation into the mechanism by which microRNA-21 induces cytokine release revealed that it suppresses the P13K/MTOR signaling pathway through targeting PTEN, resulting in increased expression and nuclear translocation of the IRF7 transcription factor driving interferon expression. These studies implicate a role and potential target for microRNAs in modulating immune responses. BioLegend provides MojoSort™ magnetic cell separation kits to aid in isolation of specific cell populations including mouse dendritic cells. Our pan dendritic cell isolation kit can be used to isolate both classical and plasmacytoid dendritic cells or in conjunction with other flow cytometry-based methods to sort specific dendritic cell subpopulations. |
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MojoSort™ Mouse Pan Dendritic Cell Isolation Kit  A single cell suspension from C57BL/6 mouse splenocytes was prepared using enzymatic digestion to isolate Pan Dendritic Cells (classical and plasmacytoid) using the MojoSort™ Mouse Pan Dendritic Cell Isolation Kit. Cells were stained with anti-mouse CD11c (clone N418) PE and anti-mouse CD317 (clone 927) APC. Dead cells were excluded by Helix NP™ Blue. |
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| Learn more about dendritic cell development, maturation and function on our dendritic cell webpage. You can also find common dendritic cell markers in both mouse and human to help you identify dendritic cell subpopulations in your research! |
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