Our antibodies are compatible with a variety of multiplexed spatial protein analysis platforms and techniques. These include both commercially available and community-developed (open) applications.

 

The following table provides a current snapshot of our reagents that have been reported to work with each platform or technique. The reported compatibility of our clones highlighted below originates from the commercial vendor or community listed for each method. Please note, since we have not verified these methods in-house, we will only be able to provide limited support on these applications.

 

 

Method

Instrument

Company

Detection Method (Read-out)

Number of Targets Per Assay (Plexity)

Recommended and Screened Targets

Supported Sample Types

Cyclic Imaging

Phenocycler/

PhenoImager

Akoya

Fluorophores

40 typical,

100 possible

206 Akoya-screened purified antibodies

 

Customer-generated  targets

 

Conjugation kits are available from Akoya

 

 

FFPE, FF

T-CycIF

Standard fluor-imagers 

Open-source (standard imaging systems)

Fluorophores

Up to 60

21 recommended antibodies (including conjugated and purified clones)

 

FFPE

ChipCytometry

CellScape/

Zellscanner

Bruker/

Canopy

Fluorophores

60

Canopy-recommended target list

 

Customers will need to validate specific clones

FFPE, FF, PBMC

SeqIF

COMET™/LabSat®

Lunaphore

Fluorophores (uses secondary fluor-conjugated antibodies)

40-60 (COMET™)

10 (LabSat®)

Qualified and verified targets from Lunaphore

 

Customers will need to validate specific primary antibody clones

FFPE, FF

Imaging Mass Cytometry

Hyperion™

(Helios™+ Imager)

Standard BioTools (formerly Fluidigm)

Metals

(mass spectrometry)

40-plex commercially available

(100-plex possible)

Conjugation kit available from Standard BioTools

 

Publication using 7 BioLegend antibodies

 

Customers will need to validate clones

 

FFPE, FF

MIBI™

MIBIScope™

Ionpath

Metals

(mass spectrometry)

40

A conjugation kit is available from Ionpath

 

Customers will need to validate clones

 

FFPE, FF

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