Description: This month’s Ken Lau Show takes a look at the issue of autofluorescence, the molecules that create it, and how you can avoid it in your samples.

Links: 

Autofluorescence blog

Autofluorescence in microscopy: 
https://www.microscopyu.com/references/pdfs/Viegas_etal_Eur_J_Histochem-51-59-2007.pdf
https://www2.cbm.uam.es/confocal/Manuales/autofluorescence.pdf

Description: With Halloween fast approaching, we look at our Zombie Fixable Viability Dyes to offer tips for its use in flow cytometry and microscopy. 

Links: 

Zombie Fixable Viability Dyes
Download the Zombie Dyes App
Panel Builder 
Tech support

Description: On this Ken Lau Show, we look at some more tips for panel building for flow cytometry, like marker expression and bivariate analysis. Keywords: flow cytometry, multicolor, panel building, bivariate, acute myeloid leukemia, expression, BioLegend, Ken Lau, science, tips, help. 

Links:

PE/Dazzle™ 594 promotion and informatiom
Flow cytometry for acute myeloid leukemia
Expression of common surface molecules on blood cells
Panel Builder
Tech support

Description: You perform flow cytometry all the time, but do you know how this technology came about? This month’s Ken Lau show takes a look at the key milestones of development for both the flow cytometer and fluorophores.

Links:

History of Flow Cytometry

Description: This month, Ken Lau hosts his very own game show! He quizzes contestants on the merits and features of BioLegend's brand new product, LEGENDplex™, which is a bead-based multi-analyte flow assay kit.

Links:

LEGENDplex™

Description: On this month's Ken Lau Show, we take a look at some considerations for panel building in nuclear antigen staining, particularly fluorophore size, and methanol/ethanol fixations.

Links:

Flow Cytometry Buffers
PE/Dazzle™ 594 Conjugates
The Tandem

Description: There are a lot of misconceptions when it comes to the use of tandem fluors in flow cytometry. This month's episode of the Ken Lau show covers the basics of how tandem works, tips for their use, and our brand new tandem, PE/Dazzle™ 594.

Links:

FluoroFix™ Buffer
PE/Dazzle™ 594 Conjugates
Tandem Dyes

Description: When performing flow cytometry, you have to have the proper controls in order to understand what's real in your results. This month's episode of the Ken Lau show looks at the importance of autofluorescence, isotype controls, and fluorescence minus one controls. 

Links:

TruStain FcX™ Products

Description: This month, we cover the fundamental building blocks useful for creating a panel. We discuss the proper controls that should be included with every experiment and how you can break your panel into easily digestible tiers for proper fluor assignment.

Links:

Fluorophore Brightness Chart
Rainbow Beads Blog
Multicolor Staining Guide
Panel Building Reference
Panel Builder
Tech Support

Description: Fluorescent proteins produce fantastic colors and serve as excellent reporters for genes of interest. In our inaugural episode, Dr. Ken Lau takes you through the origin of the first fluorescent protein and how these proteins can affect your panel’s other markers.

Links:

GFP Blog
Spectra Analyzer
Multicolor Panel Selector
Custom Panel Construction