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Alexa Fluor® 647 anti-CD31 (PECAM-1) Antibody

Pricing & Availability
Clone
JC70 (See other available formats)
Regulatory Status
RUO
Other Names
Platelet and endothelial cell adhesion molecule (PECAM-1), EndoCAM
Isotype
Mouse IgG1, κ
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Product Citations
publications
A. JC70_AF647_CD31_Antibody_1_093024
IHC staining with Alexa Fluor® 647 anti-CD31 (PECAM-1) (clone JC70) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated with (panel A) or without (panel B) Alexa Fluor® 647 anti-CD31 (PECAM-1) (clone JC70). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged (panels C and D). Scale bar: 50 μm
  • A. JC70_AF647_CD31_Antibody_1_093024
    IHC staining with Alexa Fluor® 647 anti-CD31 (PECAM-1) (clone JC70) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated with (panel A) or without (panel B) Alexa Fluor® 647 anti-CD31 (PECAM-1) (clone JC70). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged (panels C and D). Scale bar: 50 μm
  • B. JC70_AF647_CD31_Antibody_2_093024
    HEL cells (positive cell line, panels A and C) and A-431 cells (negative cell line, panels B and D) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Alexa Fluor® 647 anti-CD31 (PECAM-1) (clone JC70) (red, panels A and B). Nuclei were counterstained with DAPI (Cat. No. 422801) (blue). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged (panels C and D). Scale bar: 20 μm
  • C. JC70_AF647_CD31_Antibody_3_093024
    HEL cells (positive cell line, filled histogram) and A-431 cells (negative cell line, open histogram) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with either Alexa Fluor® 647 anti-CD31 (PECAM-1) (clone JC70) or Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (open histogram, dashed line) (representative of both cell lines) (Cat. No. 400130).
  • D. JC70_AF647_CD31_Antibody_FC_093024
    Human peripheral blood granulocytes were stained with either Alexa Fluor® 647 anti-CD31 (clone JC70) (positive cell line, filled histogram) or Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (negative control, open histogram) (Cat. No. 400130).
See Alexa Fluor® 647 spectral data
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623553 25 µg £110
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623554 100 µg £278
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Description

CD31, widely known as “platelet and endothelial cell adhesion molecule” (PECAM), is a crucial transmembrane glycoprotein primarily found on the surface of endothelial cells and platelets. The quintessential endothelial cell marker, CD31 is vital for the maintenance of vascular integrity. It plays a pivotal role in cell-cell adhesion, promoting interactions between neighboring endothelial cells, and facilitates leukocyte diapedesis during the inflammatory response. CD31 also mediates platelet-endothelial interactions, contributing to platelet aggregation and thrombus formation at sites of vascular injury. Additionally, this multifunctional molecule is involved in signal transduction pathways that govern cell migration, proliferation, and survival, making it a key player in immune response regulation and angiogenesis. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Cell membrane preparation from the spleen of a patient with hairy cell leukemia
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

ICC, ICFC, FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1 - 5 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 2.5 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

JC70 does not cross-react with mouse CD31 in Western Blot.

For ICC we recommend fixation/permeabilization with 100% ice-cold methanol, or 4% PFA followed by 100% ice-cold methanol. Fixation/permeabilization with 4% PFA followed by 0.5% Triton-X results in a significantly dimmer signal.

For IHC-P, we recommend antigen retrieval using Sodium Citrate, pH 6.0 (Cat. No. 420901). Product does not work with Tris-EDTA antigen retrieval.

Additional Product Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following:

  • Fixation buffer (Cat. No. 420801) following by 100% ice-cold methanol
  • 100% ice-cold methanol only

Antigen Details

Structure
CD31 is a 738 amino acid protein with a predicted molecular weight of 82.5 kD.
Distribution

Endothelial cells, Platelets, Granulocytes, Lymphocytes, Monocytes, Neutrophils, Cell surface

Function
Cell adhesion
Interaction
BDKRB2 and GNAQ
Ligand/Receptor
Forms homophillic interactions, also CD38, CD177, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. DeLisser HM, et al. 1994. Immunol. Today. 15:490-5.
  3. Newman PJ, et al. 1990. Science 247:1219-22.
  4. Lertkiatmongkol P, et al. 2016. Curr Opin Hematol. 23:253-9.
  5. Yeh JC, et al. 2008. Biochemistry. 47:9029-39.
Gene ID
5175 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org
Go To Top Version: 1    Revision Date: 09/30/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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