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Purified anti-MMP1 Antibody

Pricing & Availability
Clone
M4110F03 (See other available formats)
Regulatory Status
RUO
Other Names
CLG, CLGN, Interstitial collagenase, Fibroblast collagenase, MMP1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
M4110F03_Purified_MMP1_Antibody_WB_033115
Recombinant human MMP1 (Cat. No. 592902; 50 ng) treated with (lane 2) and without (lane 1) active MMP3 were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-MMP1 (clone M4110F03) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP (Cat. No. 405306) and chemiluminescence detection.
  • M4110F03_Purified_MMP1_Antibody_WB_033115
    Recombinant human MMP1 (Cat. No. 592902; 50 ng) treated with (lane 2) and without (lane 1) active MMP3 were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-MMP1 (clone M4110F03) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP (Cat. No. 405306) and chemiluminescence detection.
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675702 100 µg £157
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Description

Members of the matrix metalloproteinase family proteins (MMPs) are structurally related and zinc-containing enzymes that not only degrade the extracellular matrix (ECM) and connective tissue proteins under normal physiological setting but also during disease (e.g. arthritis and metastasis). The MMP family includes six different groups of enzymes: collagenases, gelatinases, stromelysins, transmembrane MMPs, matrilysins and others. MMPs are secreted as proenzymes that have to be cleaved in order to be activated. MMP1 consists of a pro-domain which is cleaved upon activation, a catalytic domain containing the zinc binding site, a short hinge region, and a carboxyl terminal (hemopexin-like repeats) domain. It is thought to play an important role in rheumatoid and osteo-arthritis pathogenesis as mediators of cartilage and bone destruction.

Substrates of MMP-1 include collagen I, II, III, VII, VIII, and X as well as casein, gelatin, alpha1 antitrypsin, myelin basic protein, L Selectin, pro-TNF, IL-1β, IGFBP3, IGFBP5, pro-MMP-2, and pro-MMP-9. In addition, chemokines are substrates for MMP-1. CCL2, CCL7, CCL8, and CCL13 are catalytically cleaved by MMP-1 to produce receptor antagonists. CXCL5 and CXCL12 are also substrates for MMP1. TIMPs inhibit MMPs in a 1:1 inhibitor to enzyme ratio through interaction of the N-terminal domain of the TIMP molecule with the active site of the MMP. MMP1 is overexpressed in invasive melanoma, colorectal, and esophageal cancers. Importantly, MMP-1 expression was shown to increase progressively with tumor stage.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human MMP-1.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Affinity purified.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 1.25 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2565606 (BioLegend Cat. No. 675702)

Antigen Details

Structure
Monomer, belongs to the peptidase M10A family. 469 amino acids, predicted molecular weight of 54 kD.
Distribution
Extracellular. MMP-1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes, and macrophages.
Function
MMP1 cleaves collagen of types I, II, III, VII and X. MMP-1 is thought to play an important role in the pathogenesis of rheumatoid and osteo-arthritis as mediators of cartilage and bone destruction. It is also involved in angiogenesis, tissue remodeling and cancer metastasis. MMP-1 regulates chemokine activity. MMP1 is inhibited by TIMPs, α2-macroglobulin (inhibitor of MMPs in bodily fluids). TNF-alpha; down-regulates TIMPs and enhances the expression of MMPs.
Interaction
MCP1, MCP3, MCP4, TIMP-1, MMP7, Collagen type II α1, IBP-3, Integrin α2, CD44, CD147, Brevican, Chymase 1, Aggrecan 1.
Ligand/Receptor
TIMPs.
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Enzymes and Regulators
Antigen References

1. Nagase H and Woessner JF Jr. 1999. J. Biol. Chem. 274:21491.
2. Boire A, et al. 2005. Cell 120:303.
3. Kader AK, et al. 2007. Clin. Cancer Res. 13:2614.
4. Gill SE and Parks WC. 2008. Int. J. Biochem. Cell Biol. 40:1334.
5. Mazor R, et al. 2013. J. Biol. Chem. 288:598.
6. Sommer K, et al. 2013. PLos One 8:e73992.

Gene ID
1029 View all products for this Gene ID
UniProt
View information about MMP-1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 03/31/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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