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Purified anti-α-Synuclein, aggregated Antibody

Pricing & Availability
Clone
A17183B (See other available formats)
Regulatory Status
RUO
Other Names
Non-A beta component of AD amyloid, NACP, PARK1, Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-amyloid beta component (NAP)
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A17183B_Purified_a-Synuclein_Antibody_1_032619
IHC staining of purified anti-α-Synuclein, aggregated antibody (clone A17183B) on formalin-fixed paraffin-embedded Parkinson's disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. After incubation with biotinylated anti-rat antibody, the tissue was labeled with BioLegend's HRP labeling reagent followed by addition of DAB chromogen (Cat. No. 926506) for detection and hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar 50 µm
  • A17183B_Purified_a-Synuclein_Antibody_1_032619
    IHC staining of purified anti-α-Synuclein, aggregated antibody (clone A17183B) on formalin-fixed paraffin-embedded Parkinson's disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. After incubation with biotinylated anti-rat antibody, the tissue was labeled with BioLegend's HRP labeling reagent followed by addition of DAB chromogen (Cat. No. 926506) for detection and hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar 50 µm
  • A17183B_Purified_a-Synuclein_Antibody_2_032619
    IHC staining of purified anti-α-Synuclein, aggregated antibody (clone A17183B) on formalin-fixed paraffin-embedded Parkinson's disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. After incubation with biotinylated anti-rat antibody, the tissue was labeled with BioLegend's HRP labeling reagent followed by addition of DAB chromogen (Cat. No. 926506) for detection and hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar 50 µm
  • A17183B_Purified_a-Synuclein_Antibody_3_032619
    Dot blot of purified anti-α-Synuclein, aggregated antibody (clone A17183B) on aggregated (Agg) or monomeric (Mono) recombinant human α-Synuclein. The blots were incubated with 0.1-1.0 µg/mL of the primary antibody or 1 µg/ml of Rat IgG2a isotype control for 1 hour at room temperature, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). Enhanced chemiluminescence was used as the detection system. Dot blot was used to demonstrate the specificity of this clone instead of WB.
  • A17183B_Purified_a-Synuclein_Antibody_4_032320
    IHC staining of purified anti-α-synuclein, aggregated antibody (A17183B) on formalin-fixed paraffin-embedded Parkinson’s disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 674 goat anti-rat IgG (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • A17183B_Purified_a-Synuclein_Antibody_5_032320
    IHC staining of purified anti-α-synuclein, aggregated antibody (A17183B) on formalin-fixed paraffin-embedded Parkinson’s disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 674 goat anti-rat IgG (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
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865001 25 µg £61
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865002 100 µg £165
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Description

α-synuclein is expressed principally in the nervous system, but it is also produced in the other tissues, including the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in the presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in the nuclei and mitochondria. At least three isoforms of synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms are α-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-Synuclein fibrils are a major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Aggregated recombinant human alpha-synuclein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 µg/mL for chromogenic staining and 0.1 - 2.0 µg/mL for fluorescent staining is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Kumar ST, et al. 2020. Neurobiol Dis. 146:105086. PubMed
RRID
AB_2810765 (BioLegend Cat. No. 865001)
AB_2810765 (BioLegend Cat. No. 865002)

Antigen Details

Structure
Human α-synuclein is a 140 amino acid protein with a molecular mass of 14 kD.
Distribution

Tissue distribution: Primarily nervous system, but lower expression in other tissues such as blood and skin.
Cellular distribution: Cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extracellular.

Function
α-synuclein plays several roles in synaptic activity such as synaptic vesicle trafficking and neurotransmitter release. It is also involved in the regulation of gene expression.
Interaction
Interacts with various proteins, such as UCHL1, phospholipase D, histones and SNARE components.
Cell Type
Neurons
Biology Area
Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Misfolding and Aggregation, Protein Trafficking and Clearance, Synaptic Biology
Molecular Family
α-Synuclein, Presynaptic proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Mor DE, et al. 2016. Neurobiol. Dis. 88:66.
  2. Jucker M, Walker LC. 2013. Nature. 501(7465):45.
  3. Bartels T, et al. 2011. Nature. 477(7362):107.
  4. Devine MJ, et al. 2011. Mov. Disord. 26:2160.
Gene ID
6622 View all products for this Gene ID
UniProt
View information about alpha-Synuclein aggregated on UniProt.org
Go To Top Version: 3    Revision Date: 03/23/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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