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Application Abbreviation Description
Activation Activ Induction of a cellular active state from a normal resting state.
Agonist Agonist Use of a cell-surface targeted antibody to activate a biological response. The response can be either positive or negative depending on the function of the targeted protein.
Apoptosis Apop A process of programmed cell death.
Bioassay BA Use of a recombinant protein to induce a biological response.
Blocking Block Use of a cell-surface targeted antibody to block a biological response.
Cell Culture Cell Culture Any reagent, protein, or solution suitable for use in cell culture.
Cell Screening Cell Screening Characterization and determination of the immunophenotype of a cell population.
Cell Separation Separation Separation of a defined cell population using magnetic nanoparticles either by positive or negative selection.
Cell Separation by Negative Selection Cell Sep – Neg Separation of a defined cell population using magnetic nanoparticles by negative selection, also called depletion.
Cell Separation by Positive Selection Cell Sep – Pos Separation of a defined cell population using magnetic nanoparticles by positive selection.
Chromatin Immunoprecipitation ChIP Use of an antibody to precipitate a protein-DNA complex in order to determine protein binding sites on DNA.
Complement Mediated Cell Depletion CMCD Use of an antibody to target cells for complement-mediated cell lysis.
Costimulation Costim Enhancement of a primary stimulatory signal by targeting a different molecule.
Cytotoxicity Cyt Use an antibody to trigger a cytotoxic cellular response that is not apoptotic.
Depletion Depletion In vivo, refers to injection of antibody to deplete targeted cell types. In vitro, refers to addition of an antibody in culture to deplete target cells.
Direct Enzyme-Linked Immunosorbent Assay Direct ELISA Antibody is conjugated to a detection enzyme to bind the plate-immobilized target antigen.
Electron Microscopy EM Transmission electron microscopy is a microscopy technique that detects the scatter of electrons through a thin specimen, providing higher resolution images than other microscopy techniques.
Enzyme-Linked Immunosorbent Assay ELISA Enzyme-linked Immunosorbent Assay
- LEGEND MAX™ Kits include: Pre-coated plates, detection antibody, recombinant standards, avidin-HRP, and all the required buffers.
- ELISA MAX™ Deluxe sets include: ELISA Plates, Capture and detection antibodies, recombinant standards, avidin-HRP, assay diluents, coating buffer, TMB substrate.
- ELISA MAX™ Standard sets include:  Capture and detection antibodies, recombinant standards, and avidin-HRP.
ELISPOT ELISPOT Enzyme-linked Immunosorbent Spot
Functional Assay FA Use of a cell-surface targeted antibody to induce, modify, or block a biological response. Can also be applied to the use of a product to measure a functional activity within a cell.
Flow Cytometry FC Flow cytometric analysis of antibody surface-stained cells.
Intracellular Staining for Flow Cytometry ICFC Flow cytometric analysis of intracellularly-stained cells.
Immunocytochemistry ICC Immunocytochemistry is a technique used to assess the presence of a specific protein or antigen in cells (cultured cells, cell suspensions) by use of specific antibodies, thereby allowing visualization and examination under a microscope. The readout can be colorimetric using chromogen substrate or fluorescent using fluorophore-conjugated antibodies.
Immunofluorescence Microscopy IF Microscopic examination of fluorescently-labeled cells, either grown on or spun onto slides, or tissue preparations.
Immunohistochemistry IHC Analysis of antibody-labeled tissue preparations, typically frozen or paraffin-embedded.
3D Immunohistochemistry 3D IHC Immunodetection applied to formalin-fixed tissue sections of at least 300 microns in thickness, which are optically cleared by tissue clearing reagent.
Immunohistochemistry - Frozen IHC-F Immunodetection applied to frozen tissues (fluorescent or chromogenic imaging)
Immunohistochemistry - Paraffin IHC-P Immunodetection applied to formalin-fixed paraffin-embedded (FFPE) tissues (fluorescent or chromogenic imaging)
Immunoprecipitation IP Precipitation of a target protein from solution using an antibody.
Intracellular Proteogenomics ICPG Antibody oligonucleotide conjugates designed to enable the generation of intracellular proteomic data, using single cell devices such as 10x Genomics' Chromium platform and Illumina sequencers.
Knockout/Knockdown target confirmation by Western Blot KO/KD-WB Antibody specificity validation by Western Blot using lysates derived from Knockout or Knockdown of target gene by CRISPR/Cas9 or siRNA
Live cell imaging Live cell imaging Reagents that are applied to and imaged in living cells or tissue explants.
Mass Cytometry CyTOF® Single cell analysis of antigen expression using a novel elemental mass-spectrometry detection technology (Inductively Coupled Plasma Mass Spectrometer, ICP-MS) that utilizes isotope-labeled antibodies.
Multiplex bead-based assay Multiplex A flow cytometry based assay in which beads with differing internal levels of fluorescence and size are used to capture specific analytes using a sandwich ELISA type quantification.
Neutralization Neut Neutralization of a soluble factor by an antibody.
Proteogenomics PG Antibody oligonucleotide conjugates designed to enable the generation of transcriptomic and proteomic data simultaneously, using single cell devices such as 10x Genomics' Chromium and Illumina sequencers.
Purification Purification A process designed to enrich a single type of protein or particle from a heterogenous and complex mixture/supernatant.
RIA RIA A very sensitive in vitro assay technique used to measure concentrations of antigens (for example, hormone levels in the blood) by use of antibodies and radioactive materials.
Spatial Biology SB

Multiomic or multiplexed analysis of tissue samples in their spatial context. The readout can be sequencing-based, fluorescent, or another method to detect conjugated antibodies.

- Verified: application is verified in-house.

- Reported in the literature, not verified in-house: application is reported in research literature.

- Community verified: application is verified by collaborators and partners.
Stimulation Stim Induction of a measurable cellular response to external stimuli, such as an antibody.
Western Blotting WB Use of gel electrophoresis to separate proteins by size, followed by detection with an antibody.

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