We developed our new Cyto-Fast™ Fix/Perm Buffer Set after rigorous in-house testing to determine optimal buffer formulations for cytokine staining. Advantages of the Cyto-Fast™ Fix/Perm Buffer set include:

  • Improved signal-to-noise for staining of cytokines and other intracellular proteins like Granzyme B and Perforin.
  • Maintains signal intensity of surface antibody stains.
  • Fixation and permeabilization buffers are sold and quality tested as a set.
Description: Size Cat. No.
300 tests 426803

Samples Available! Contact sales@biolegend.com for more information.

PMA + ionomycin stimulated (3 hours) human peripheral blood lymphocytes (in the presence of monensin) were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer Set. Cells were then stained with antibodies as indicated.

If you’re looking for a way to reliably quantify soluble cytokines, chemokines, or biomarkers in your sample, try one of our ELISA kits.

Our newest kit allows you to quantitate uncarboxylated osteocalcin from cell culture supernatant, serum, and plasma. Osteocalcin is abundant in the bone in a carboxylated form. Uncarboxylated forms of the protein are released into the blood and act as hormones that are involved in insulin sensitivity and glucose tolerance. Learn more about the kit...
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