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| Many chronic autoimmune disorders such as systemic lupus erythematosus, psoriasis, and Sjögrens syndrome are associated with an overactive Th17 response. While ACT1 (IL-17R adaptor protein) plays a role in mediating downstream IL-17 signaling, loss of ACT1 results in spontaneous autoimmune diseases. Zhang et al. attached the DYKDDDDK epitope tag to STAT3 and the V5 or HA-epitope tag to ACT1. This allowed them to demonstrate how ACT1 directly interacts with STAT3 to inhibit downstream signaling. BioLegend offers a wide variety of epitope tag antibodies and associated reagents to help study direct protein-protein interactions and how point mutations may alter these interactions and downstream signaling events. |
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| Adapted from Zhang et al. 2018. Nature Communications. 16:9. Pubmed. |
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 Learn more about how using our Direct-Blot™ antibodies can save you time by eliminating the need for a secondary antibody! |
| Alexa Fluor® 488 anti-DYKDDDDK Tag Antibody |
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| HeLa cells non-transfected (A) or transfected with DYKDDDDK Tag fused protein (B and C) were fixed, permeabilized, and blocked. Then the cells were intracellularly stained overnight at 4°C with 1: 250 diluted (2 μg/ml, Fig. A and B) and 1: 1000 diluted (0.5 μg/ml) Alexa Fluor® 488 anti-DYKDDDDK Tag (Fig, C, green). Nuclei were counterstained with DAPI (blue). |
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| We've recently updated our immunologic networks poster providing a comprehensive overview of both the innate and adaptive branches of immunology. Request your own copy or take a narrated tour through the poster in our 360 video! |
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| Dendritic cells are key antigen presenting cells that initiate and control lymphpoid cell responses. Learn more on our newly updated Dendritic Cell webpage featuring more information about dendritic cell development, phenotyping markers, and myeloid cells in the CNS. |
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