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A single cell suspension from C57BL/6 mouse bone marrow was prepared to isolate monocyte cells using the MojoSort™ Mouse Monocyte Isolation Kit. Cells were stained with Lin cocktail CD3ε (145-2C11)/ CD45R(RA3-6B2)/ CD49b(DX5)/ Ly-6G(1A8)/ NK1.1(PK136)/ Siglec-F(S17007L)-APC and CD11b (M1/70)-PE. Dead cells were excluded by Helix NP™ Blue. -
A single cell suspension from C57BL/6 mouse bone marrow was prepared to isolate monocyte cells using the MojoSort™ Mouse Monocyte Isolation Kit. Cells were stained with Ly-6C (clone HK1.4) Pacific Blue™ and CX3CR1 (clone SA011F11) APC. Dead cells were excluded by Helix NP™ Blue.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 480153 | 10 tests | 209€ | ||||
| 480154 | 100 tests | 620€ | ||||
Non-monocyte cells are depleted by incubating the sample with the Mouse Monocyte Isolation biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched mouse monocyte cells are collected by decanting the liquid in a clean tube. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Kit Contents
- Kit Contents
-
For Cat. No. 480153:
- 100 µL Biotin-Antibody Cocktail
- 100 µL Streptavidin Nanobeads
For Cat. No. 480154:
- 1 mL Biotin-Antibody Cocktail
- 1 mL Streptavidin Nanobeads
Product Details
- Verified Reactivity
- Mouse
- Formulation
-
Cocktail: Phosphate buffer solution containing 0.09% sodium azide, 0.5% BSA, pH 7.2.
Particle: Aqueous solution containing BSA and 0.05% sodium azide. - Preparation
-
The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Streptavidin Nanobeads: Streptavidin-coated magnetic beads. - Storage & Handling
- All components should be stored undiluted between 2°C and 8°C.
- Application
- Recommended Usage
-
10 µL of Biotin-Antibody Cocktail for 1 x 107 cells in 100 µL of buffer
10 µL of Streptavidin Nanobeads for 1 x 107 cells in 100 µL of buffer - Application Notes
-
This kit is designed for the isolation of untouched mouse monocyte cells from bone marrow.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
Antibody or cocktail dilution to use in column: 8X
Nanobead dilution to use in column: 6X
Antigen Details
- Gene ID
- NA
- Specificity (DOES NOT SHOW ON TDS):
- Monocyte
- Specificity Alt (DOES NOT SHOW ON TDS):
- Monocyte
- App Abbreviation (DOES NOT SHOW ON TDS):
- Cell Sep - Neg
- UniProt
- View information about Monocyte on UniProt.org
Related FAQs
- Is there a way to detach your magnetic particles from the cell surface?
-
MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.
MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.
- What is the size of your magnetic particles?
-
MojoSort Nanobeads: the average diameter is approximately 130 nm.
MojoSort Microbeads: the average diameter is approximately 1 μm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
-
MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- What antibodies are present in the depletion cocktails provided for isolation kits?
-
Please contact our technical service team for further assistance.
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