- Clone
- GB11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Granzyme-2, serine protease B, CCP1, Asp-ase, CTLA-1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
515409 | 25 tests | 159€ | ||||
515410 | 100 tests | 344€ |
Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Reported Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application References
-
- Wever PC, et al. 1998. Immunology. 93:383
- Arens R, et al. 2004. J. Exp. Med. 199:1595
- Lima M, et al. 2003. Am. J. Pathol. 163:763
- Wiede F, et al. 2014. J Autoimmun. 53:105. PubMed
- Baker GF, et al. 2014. Cancer Res. 74:5079. PubMed
- Nacer A, et al. 2014. PLoS Pathog. 10:1004528. PubMed
- Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed
- RRID
-
AB_3106190 (BioLegend Cat. No. 515409)
AB_3106190 (BioLegend Cat. No. 515410)
Antigen Details
- Structure
- 32 kD serine protease
- Distribution
-
Cytotoxic T-cells and NK cells, low on granulocytes, B cells and activated dendritic cells
- Function
- Induction of cell death and apoptosis
- Ligand/Receptor
- Mannose-6-phosphate receptor
- Cell Type
- B cells, Dendritic cells, NK cells, T cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience
- Molecular Family
- Enzymes and Regulators, Proteases
- Antigen References
-
1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203
2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885
3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610
4. Wagner C, et al. 2008. Mol. Immunol. 45:1761 - Gene ID
- 3002 View all products for this Gene ID 14939 View all products for this Gene ID 171528 View all products for this Gene ID
- UniProt
- View information about Granzyme B on UniProt.org
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
-
It is lot-specific. On average it ranges between 2-4.
Other Formats
View All Granzyme B Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
FITC anti-human/mouse Granzyme B | GB11 | ICFC |
Alexa Fluor® 647 anti-human/mouse Granzyme B | GB11 | ICFC |
Pacific Blue™ anti-human/mouse Granzyme B | GB11 | ICFC |
Brilliant Violet 421™ anti-human/mouse Granzyme B | GB11 | ICFC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
FITC anti-human/mouse Granzyme B
Human peripheral blood lymphocytes surface stained with CD8 ... Human peripheral blood lymphocytes surface stained with CD8 ... -
Alexa Fluor® 647 anti-human/mouse Granzyme B
Human peripheral blood lymphocytes were surface stained with... C57BL/6 mouse splenocytes were stimulated with plate-bound a... -
Pacific Blue™ anti-human/mouse Granzyme B
Human peripheral blood lymphocytes were surface stained with... -
Brilliant Violet 421™ anti-human/mouse Granzyme B
Human peripheral blood lymphocytes were surface stained with...
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