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Purified anti-GRB2 Antibody

Pricing & Availability
Clone
W17230A (See other available formats)
Regulatory Status
RUO
Other Names
Growth Factor Receptor Bound Protein 2, SH2/SH3 Adaptor Protein GRB2, ASH, Epidermal Growth Factor Receptor-Binding Protein GRB2, Abundant SRC Homology, MSTP084, Grb3-3, MST084, NCKAP2
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W17230A_PURE_GRB2_Antibody_062519
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-GRB2 antibody, clone W17230A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • W17230A_PURE_GRB2_Antibody_062519
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-GRB2 antibody, clone W17230A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
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627001 25 µg $101
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627002 100 µg $253
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Description

GRB2 is a receptor tyrosine kinase (RTK) adaptor protein that transduces signaling cascades from activated receptors. Upon receptor stimulation, GRB2 binds phosphorylated RTK tyrosine residues via a central SH2 domain; flanking GRB2 SH3 domains facilitate the recruitment of downstream effectors to the activated RTK.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Synthetic peptide corresponding to amino acid residues 199-214 of human GRB2.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone has not been tested for ICC or IP.

RRID
AB_2810687 (BioLegend Cat. No. 627001)
AB_2810687 (BioLegend Cat. No. 627002)

Antigen Details

Structure
GRB2 is a 217 amino acid protein with a predicted molecular weight of 25 kD.
Distribution

Plasma membrane-associated/ubiquitously expressed

Function
Adaptor protein in RTK signaling pathways
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Adaptor Proteins, Phospho-Proteins
Antigen References
  1. Lowenstein EJ, et al. 1992. Cell. 70:431-42.
  2. Matuoka K, et al. 1992. Proc Natl Acad Sci U S A. 89:9015-9.
  3. Skolnik EY, et al. 1993. Science. 260:1953-5.
  4. Pendergast AM, et al. 1993. Cell. 75:175-85.
Gene ID
2885 View all products for this Gene ID
UniProt
View information about GRB2 on UniProt.org
Go To Top Version: 2    Revision Date: 10/29/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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