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Purified anti-mouse Panendothelial Cell Antigen Antibody

Pricing & Availability
Clone
MECA-32 (See other available formats)
Regulatory Status
RUO
Other Names
Pan-endothelial Cell Antigen, MECA-32, Plasmalemma vesicle-associated protein
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
MECA-32_A488_061109.jpg
bEND.3 cells stained with MECA-32 Alexa Fluor® 488
  • MECA-32_A488_061109.jpg
    bEND.3 cells stained with MECA-32 Alexa Fluor® 488
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Cat # Size Price Quantity Check Availability Save
120501 50 µg $71
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120502 500 µg $262
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Description

MECA-32 is a 50-55 kD homodimer glycoprotein primarily expressed on most endothelial cells in the embryonic and the adult mouse tissues, except in the brain and in skeletal and cardiac muscle, where it has more restricted distribution. In skeletal and cardiac muscle, the MECA-32 antigen is confined in small arterioles and venules. But in some conditions, such as inflammation or cardiac transplantation, MECA-32 expression can be induced on some negative-cardiac endothelial cells. In the brain, its expression occurs only in the circumventricular organs and the neurohemal tissues. During embryonic development, MECA-32 antigen is expressed on the brain vasculature up to day 16 of gestation, and disappears at day 17 and after.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse lymph node stromal cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, IHC-F, IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (in the relevant formats) include: immunoprecipitation, Western blotting, immunocytochemistry, and immunohistochemical staining of acetone-fixed frozen sections2-5. Clone MECA-32 is not recommended for formalin-fixed paraffin sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Leppink DM, et al. 1989. Transplantation 48:874.
  2. Bergese SD, et al. 1994. Transplantation 57:711. (IHC)
  3. Tanneau GM, et al. 1999. J. Histochem. Cytochem. 47:1581. (IHC)
  4. Kopp HG, et al. 2005. Blood 106:505. (IHC)
  5. Hallmann R, et al. 1995. Dev. Dyn. 202:325. (IHC)
Product Citations
  1. Lopez-Dee Z, et al. 2015. PLoS One. 10: e0139918. PubMed
  2. Alfaro D, et al. 2015. J Leukoc Biol. 98: 883 - 896. PubMed
  3. Engelbrecht E, et al. 2022. Microcirculation. 29:e12752. PubMed
  4. Frenkel N, et al. 2023. Front Oncol. 13:1062926. PubMed
  5. Nakagawa T, et al. 2021. Cell Rep. 37:109875. PubMed
  6. Chee J, et al. 2011. J Immunol. 187:1702. PubMed
  7. Zheng J, et al. 2011. Blood. 117:470. PubMed
  8. Qiao J, et al. 2015. Sci Rep. 5: 17828. PubMed
  9. Scott NA, et al. 2018. Front Immunol. 2.319444444. PubMed
  10. Shono Y, et al. 2010. Blood. 115:5401. PubMed
  11. Yang B, et al. 2018. J Am Soc Nephrol. 29:1900. PubMed
RRID
AB_493302 (BioLegend Cat. No. 120501)
AB_493302 (BioLegend Cat. No. 120502)

Antigen Details

Structure
50-55 kD homodimer
Distribution

Endothelial cell in the embryonic and the adult mice, except in the brain and in skeletal and cardiac muscle, where it has more restricted distribution

Cell Type
Embryonic Stem Cells, Endothelial cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules
Antigen References

1. Leppnik DM, et al. 1989. Transplantation 48:874.
2. Hallmann R, et al. 1995. Dev. Dyn. 202:325.
3. Kruse A, et al. 1999. Bio. Reprod. 61:1393.

Gene ID
84094 View all products for this Gene ID
UniProt
View information about Panendothelial Cell Antigen on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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