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- Regulatory Status
- RUO
- Other Names
- CD105, CD105 Antigen, ENG, ORW, ORW1, HHT1, END, Osler-Rendu-Weber syndrome 1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Human Endoglin inhibits the alkaline phosphatase production induced by human BMP10 in ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.12 – 0.72 µg/mL. -
Stability Testing for recombinant human Endoglin. Recombinant human Endoglin was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested for their ability to inhibit the production of alkaline phosphatase induced by human BMP10 in ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 0.12 – 0.72 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 576714 | 25 µg | $380 | ||||
| 576716 | 100 µg | $790 | ||||
Endoglin (ENG; also known as CD105) is a type I transmembrane glycoprotein located on cell surfaces. ENG is expressed by vascular endothelial cells, syncytiotrophoblasts of placenta, pro-fibrogenic cells and hepatic stellate cells. ENG is weakly expressed on stromal fibroblasts. It is also expressed on activated monocytes and tissue macrophages. Expression of ENG is increased on activated endothelium in tissues undergoing angiogenesis, such as in tumors, wound healing or dermal inflammation. ENG functions as a coreceptor for ligands of the TGFβ superfamily. ENG is a component of the TGF-β receptor system in human umbilical vein endothelial cells and binds TGF-β1 and β3 with high affinity but does not bind to TGF-β2. ENG plays important roles in endothelial proliferation, cardiovascular development, tumor growth/survival, and metastasis of cancer cells. Mutations in the ENG gene lead to Hereditary Hemorrhagic Telangiectasia, a disorder characterized by arteriovenous malformations. An extracellular domain of the full-length membrane ENG, soluble ENG, is a proposed biomarker of cardiovascular disease progression, cardiovascular disease treatment and endothelial dysfunction. Furthermore, ENG is overexpressed in the tumor-associated vascular endothelium, where it modulates angiogenesis.
Product DetailsProduct Details
- Source
- Recombinant human Endoglin, amino acid Glu26-Gly586 (Accession No. P17813.2) with a C-terminal hIgG1 Fc tag was expressed in CHO cells.
- Molecular Mass
- The 800 amino acid recombinant protein has a predicted molecular mass of approximately 87.6 kD. The DTT-reduced and non-reduced proteins migrate at approximately 90 - 115 kD and 200 – 240 kD by SDS-PAGE respectively. The N-terminal amino acid is Glu.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Human Endoglin inhibits the alkaline phosphatase production induced by human BMP10 (Cat. No. 751604) in a dose-dependent manner. The ED50 = 0.12 – 0.72 µg/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
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Endothelial cells, activated monocytes/macrophages, bone marrow stromal cells, hematopoietic stem/progenitor cells
- Function
- Modulates cellular response to TGF-β, involved in vascular development and remodeling
- Interaction
- TGFBR1, TGFBR2, TGF-beta 1, TCTEX1D4, ARRB2 and GDF2
- Ligand/Receptor
- TGF-β1, TGF-β3
- Bioactivity
- Endoglin inhibits the alkaline phosphatase production induced by human BMP10 in ATDC5 mouse chondrogenic cells.
- Cell Type
- B cells, Endothelial cells, Leukocytes, Neutrophils, T cells
- Biology Area
- Angiogenesis, Cell Adhesion, Cell Motility/Cytoskeleton/Structure, Immuno-Oncology, Immunology, Signal Transduction
- Molecular Family
- CD Molecules
- Antigen References
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- Rathouska J, et al. 2015. Atherosclerosis. 243:383-8.
- Kapur NK, et al. 2013. Vasc. Health Risk Manag. 9:195-206.
- Meurer SK, et al. 2014. World J Biol Chem. 5:180-203
- Gregory AL, et al. 2014. Placenta. 35:S93-9.
- Nassiri F, et al. 2011. Anticancer Res. 31:2283.
- Zhang Y, et al. 2011. Int J Clin Exp. Med. 4:32-42.
- Gene ID
- 2022 View all products for this Gene ID
- UniProt
- View information about Endoglin on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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