Mouse

Cytokine/Chemokine	Clone(s)	Target Cells	Stimulation	Stimulation period	Protein transport blocker	Surface marker
IL-1α	ALF-161	Thioglycollate-elicited peritoneal macrophage	LPS (1 μg/ml)	4-6 hours	monensin or brefeldin A	CD107b (MAC-3)
IL-2	JES6-5H4	Splenocytes	PMA (20 ng/ml) plus ionomycin (1μg/ml)	4-6 hours	monensin or brefeldin A	CD3
IL-3	MP2-8F8	Purified CD4+ T cells stimulated with immobilized anti-CD3 (10 μg/ml) + soluble anti-CD28 (2 μg/ml) + IL-2 (20 ng/ml) + IL-4 (50 ng/ml) for 2 days, wash and then culture in IL-2 + IL-4 for 3-4 days	PMA (20 ng/ml) plus ionomycin (1 μg/ml)	4-6 hours	monensin or brefeldin A	CD4*
IL-4	11B11	Purified CD4+ T cells stimulated with immobilized anti-CD3 (10 μg/ml) + soluble anti-CD28 (2 μg/ml) + IL-2 (20 ng/ml) + IL-4 (50 ng/ml) for 2 days, wash and then culture in IL-2 + IL-4 for 3-4 day	PMA (20 ng/ml) plus ionomycin (1 μg/ml)	4-6 hours	monensin or brefeldin A	CD4*
IL-5	TRFK	Purified CD4+ T cells stimulated with immobilized anti-CD3 (10 μg/ml) + soluble anti-CD28 (2 μg/ml) + IL-2 (20 ng/ml) + IL-4 (50 ng/ml) for 2 days, wash and then culture in IL-2 + IL-4 for 3-4 day	PMA (20 ng/ml) plus ionomycin (1 μg/ml)	4-6 hours	monensin or brefeldin A	CD4*
IL-6	MP5-20F3	Thioglycollate-elicited peritoneal macrophage	LPS (1 μg/ml)	4-6 hours	monensin or brefeldin A	CD107b (MAC-3)
IL-9	RM9A4	Purified CD4+ T cells stimulated with immobilized anti-CD3 (5 μg/ml) + soluble anti-CD28 (5 μg/ml) + IL-2 (20 ng/ml) + IL-4 (10 ng/ml) + TGF-β1 (10 ng/mL) + anti-mouse IFN-γ (10 μg/ mL) for 3 days	PMA (500 ng/ml) plus ionomycin (500 ng/ml)	4-6 hours	Brefeldin A	CD4*
IL-10	JES5-16E3	Purified CD4+ T cells stimulated with immobilized anti-CD3 (10 μg/ml) + soluble anti-CD28 (2 μg/ml) + IL-2 (20 ng/ml) + IL-4 (50 ng/ml) for 2 days, wash and then culture in IL-2 + IL-4 for 3-4 day	PMA (20 ng/ml) plus ionomycin (1 μg/ml)	4-6 hours	monensin or brefeldin A	CD4*
IL-12	C15.6	Thioglycollate-elicited peritoneal macrophage	IFN-γ (10 ng/ml) prime for 2 hours, then add LPS (1 μg/ml)	18-24 hours	monensin or brefeldin A	CD107b (MAC-3)
IL-17A	TC11- 18H10.1	Purified CD4+ T cells stimulated with immobilized anti-CD3 (2 μg/ml) + soluble anti-CD28 (5 μg/ml), IL-6 (50 ng/ mL), TGF-β1 (1ng/mL), anti-mouse IL-4 (10 μg/mL), anti-mouse IFN-γ (10 μg/ mL), and 300 nM of FICZ cultured for 3 days. Alternatively, mouse IL-23 (5 ng/ ml) can be used in place of FICZ.	PMA (500 ng/ml) + ionomycin (500 ng/ml)	4-6 hours	Brefeldin A	CD4*
IL-17F	9D3.1C8	Purified CD4+ T cells stimulated with immobilized anti-CD3 (2 μg/ml) + soluble anti-CD28 (5 μg/ml), IL-6 (50 ng/ mL), TGF-β1 (1ng/mL), anti-mouse IL-4 (10 μg/mL), anti-mouse IFN-γ (10 μg/ mL), and 300 nM of FICZ cultured for 3 days. Alternatively, mouse IL-23 (5 ng/ ml) can be used in place of FICZ.	PMA (500 ng/ml) + ionomycin (500 ng/ml)	4-6 hours	Brefeldin A	CD4*
IL-21	BL25168	Purified CD4+ T cells stimulated with immobilized anti-CD3 (2 μg/ml) + soluble anti-CD28 (5 μg/ml), IL-6 (50 ng/ mL), TGF-β1 (1ng/mL), anti-mouse IL-4 (10 μg/mL), anti-mouse IFN-γ (10 μg/ mL), and 300 nM of FICZ cultured for 3 days. Alternatively, mouse IL-23 (5 ng/ ml) can be used in place of FICZ.	PMA (500 ng/ml) + ionomycin (500 ng/ml)	4-6 hours	Brefeldin A	CD4*
IL-22	Poly5164	Purified CD4+ T cells stimulated with immobilized anti-CD3 (2 μg/ml) + soluble anti-CD28 (5 μg/ml), IL-6 (50 ng/ mL), TGF-β1 (1ng/mL), anti-mouse IL-4 (10 μg/mL), anti-mouse IFN-γ (10 μg/ mL), and 300 nM of FICZ cultured for 3 days. Alternatively, mouse IL-23 (5 ng/ ml) can be used in place of FICZ.	PMA (500 ng/ml) + ionomycin (500 ng/ml)	4-6 hours	Brefeldin A	CD4*
IL-27	MM27-7B1	Thioglycolate-elicited Balb/c peritoneal macrophages primed with IFN-γ (100 ng/ml) for 2 hours	LPS (1 μg/ml)	4-6 hours	monensin or brefeldin A	CD107b (MAC-3)
GM-CSF	MP1-22E9	Splenocytes stimulated with immobilized anti-CD3 (10 μg/ml) + soluble anti-CD28 (2 μg/ml) for 2 days, wash and then culture in IL-2 (20 ng/ml) + IL-4 (50 ng/ml) for 3-4 day	PMA (20 ng/ml) plus ionomycin (1 μg/ml)	4-6 hours	monensin or brefeldin A	CD3
IFN-γ	XMG1.2	Splenocytes	PMA (20 ng/ml) plus ionomycin (1 μg/ml)	4-6 hours	monensin or brefeldin A	CD3
MCP-1	2H5	Thioglycollate-elicited peritoneal macrophage	LPS (1 μg/ml)	4-6 hours	monensin or brefeldin A	CD107b (MAC-3)
TNF-α	MP6-XT22	Splenocytes	PMA (20 ng/ml) plus ionomycin (1 μg/ml)	4-6 hours	monensin or brefeldin A	CD3