SARS-CoV-2 Workflow

Click on a section in the image to scroll down to your area of interest.

Featured ELISA Kits and Sets

SARS-CoV-2 Spike Protein Antibodies

SARS-CoV-2 Nucleocapsid Protein Antibodies

SARS-CoV-2 Nucleocapsid Protein

Interferons

Cytokines/Chemokines

Featured LEGENDplex™ Panels

View more products for LEGENDplex™ and ELISAs.

Cellular Responses and Anti-Viral Pathways

Once you’ve studied the entry points and how SARS-CoV-2 attacks cells, you’re ready to shift your focus onto the immune cell response. In order to gauge an effective immune response, it is critical to characterize the cellular immune response, particularly T cells. We have developed unique tools: MojoSort™ kits for the isolation of desired populations; MHC monomers pre-loaded with SARS-CoV-2 peptides for antigen-specific T cell studies; and antibodies to detect key components of anti-viral pathways. BioLegend provides key tools for discovering how cells work together to dismantle
SARS-CoV-2.

Our SARS-CoV-2 Reactive T cell Kit offers a flow-based solution for detecting T cells activated in response to SARS-CoV-2 peptide stimulation through detection of activated LFA-1 (CD11a/CD18) on the cell surface.

This comprehensive kit comes with lyophilized antibodies, positive controls (including a SARS-CoV-2 peptide pool), and critical buffers needed to complete the experiment. Each test is conveniently packaged in a single pouch, facilitating a simplified workflow. It also provides a backbone of a multicolor antibody cocktail for gating CD4⁺, CD8⁺ T cells. All of the analyzed markers are on the cell surface, so cell fixation/permeabilization procedures that can lead to significant cell loss and increased assay time are not required.

Representative Data

SARS-CoV-2 Reactive T cell Kit used on whole blood samples collected from a patient with no prior history or without vaccination against SARS-CoV-2 (negative control, top row) or vaccinated against SARS-CoV-2 (SARS-CoV-2, bottom row). From left to right: specific gating on CD4⁺ and CD8⁺ events to identify LFA-1⁺ cells within each respective population. Partial data set is shown here; full representative data is available on product details page.

Our new SARS-CoV-2-specific Flex-T™ MHC reagents simplify your protocol and save time. Each HLA monomer comes ready-to-use with fixed relevant SARS-CoV-2 peptides, removing the need for a peptide exchange step and allowing you to track T cells for potential vaccine candidates. Learn more with our Flex-T™ webpage.

Recombinant Cytokines and Growth Factors are critical for eliciting cellular responses, including proliferation, differentiation, and cytokine production. Explore our recombinant proteins, which include many key cytokines in the host cell response to SARS-CoV-2. 

We offer many antibodies to detect important mediators involved in anti-viral pathways, including Toll-like receptors, STATs, NF-κB, and interferon regulatory factors. Our anti-viral pathway reagents have been validated in applications like western blotting, flow cytometry, functional assays, ChIP, and microscopy.

Featured Products

Our comprehensive flow cytometry portfolio will help characterize any cell type, including those relevant to study anti-viral and SARS-CoV-2 immune responses. For those looking to detect various classes of human immunoglobulins, we also offer a selection of anti-human secondary reagents, which could be used to detect virus specific antibodies.

Biomarker Discovery and Monitoring

TotalSeq™ Reagents for Proteogenomics can be used to characterize single cells in a high throughput manner. These antibody-oligo conjugates contain unique nucleotide barcodes that integrate seamlessly into existing workflows, enabling you to simultaneously analyze protein and RNA expression on a single cell level. TotalSeq™ is available as individual or pre-titrated, lyophilized antibody cocktails, or hashtags for multiplexing samples. Specifically, they can be used to enable:

LEGENDScreen™ kits offer a rapid screening method for cellular samples. Kits include several 96-well plates with each well containing a different lyophilized PE-conjugated antibody useful for staining cells. Our human kit contains antibodies for 361 cell surface markers and 10 isotype controls). Our mouse kit contains 255 antibodies for cell surface markers and 11 isotype controls. View our LEGENDScreen™ products.

References:

1. Zhou, P. et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020. 579: 270.
    
2. Hoffmann, M. et al. SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell. 2020. 181:1.
    
3. Gordon, D.E. et al. A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug Repurposing. bioRxiv. 2020.