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Purified anti-GFAP Antibody

Pricing & Availability
Clone
2E1.E9 (See other available formats)
Regulatory Status
RUO
Other Names
Glial fibrillary acid protein
Isotype
Mouse IgG2b
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Product Citations
publications
1_2E1pointE9_PURE_GFAP_Antibody_1_WB_032117
Total cell lysate from HeLa (negative control), U87-MG and tissue lysate from mouse brain (15 µg/lane) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose membrane, and probed with 0.2 µg/mL of purified anti-GFAP. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection system. Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (lower). Lane M is the molecular weight ladder.
  • 1_2E1pointE9_PURE_GFAP_Antibody_1_WB_032117
    Total cell lysate from HeLa (negative control), U87-MG and tissue lysate from mouse brain (15 µg/lane) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose membrane, and probed with 0.2 µg/mL of purified anti-GFAP. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection system. Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (lower). Lane M is the molecular weight ladder.
  • 2_2E1pointE9_PURE_GFAP_Antibody_2_031920
    U251 cells were fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for five minutes and blocked with 5% FBS for 30 minutes. Then, the cells were intracellularly stained with 5 µg/mL anti-GFAP (clone 2E1.E9) antibody followed by Alexa Fluor® 594 Goat anti-mouse IgG (minimal x-reactivity) Antibody (red) for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • 3_2E1dotE9_PURE_GFAP_Antibody_IHC-F_020818
    C57BL/6 mouse frozen brain section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of anti-GFAP (clone 2E1.E9) purified overnight at 4°C, followed by 2.5 µg/ml of anti-mouse IgG2b (clone RMG2b-1) Alexa Fluor® 594 (red) for 2 hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • 4_2E1dotE9_PURE_GFAP_Antibody_IHC-P_1_092523
    IHC staining of Purified anti-GFAP (clone 2E1.E9) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902), the tissue was incubated with 5 µg/mL of purified anti-GFAP (clone 2E1.E9) followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 20X (panel A) and 40X (panel B) objective. Scale bar: 25 µm
  • 5_2E1dotE9_PURE_GFAP_Antibody_IHC-P_2_092523
    IHC staining of purified anti-GFAP (clone 2E1.E9) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Tris-EDTA pH 9.0 buffer (10 mM Tris, 1 mM EDTA), the tissue was incubated with 2 µg/mL of purified anti-GFAP (clone 2E1.E9) followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) (panel A) for 1 hour at room temperature. Tissue was also stained with 2 µg/mL of Alexa Fluor® 594 anti-Tubulin β 3 (Cat. No. 657408) (green), and nuclei were counterstained with DAPI (Cat. No. 422801) (blue). Merged images (panel B). Images were captured with a 20X objective. Scale bar: 50 µm
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644701 25 µg 124 CHF
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644702 100 µg 293 CHF
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Description

GFAP is expressed exclusively in astrocytes in the central nervous system. The protein is a member of the intermediate filament family of proteins which form networks providing support and strength to cells. This antibody does not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin and others. More than 50 GFAP mutations have been identified to be associated with the Alexander disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Bovine spinal cord homogenate
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-F, IHC-P - Verified
FC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.25 - 1.0 µg antibody per mL antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/mL is recommended. For immunohistochemical staining of frozen tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For Immunohistochemistry, Citrate buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 is recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. McLendon RE and Bigner DD. 1994. Brain Pathol. 4:221.
  2. Liu W, et al. 2011. Proteomics 11:3556. (FC) PubMed
Product Citations
  1. Liu D, et al. 2022. Eur J Neurosci. 56:3553. PubMed
  2. Whitmore CA, et al. 2022. Pharmaceuticals (Basel). 15: . PubMed
  3. Mogas Barcons A, et al. 2022. Materials (Basel). 16: . PubMed
  4. Yamaguchi N, et al. 2023. IBRO Neurosci Rep. 14:253. PubMed
  5. Salgado B, et al. 2023. Microorganisms. 11:. PubMed
  6. Tao W, et al. 2020. EMBO Mol Med. 12:e12291. PubMed
  7. Eisemann T, et al. 2018. BMC Cancer. 18:103. PubMed
  8. Liu CL, et al. 2018. Mol Med Rep. 9:2359. PubMed
  9. Gerasimov E, et al. 2021. Int J Mol Sci. 22:. PubMed
  10. Shi Y, et al. 2016. Cell Death Differ. 10.1038/cdd.2016.110. PubMed
  11. Gómez-Gálvez Y, et al. 2020. J Neurosci Res. 1417:98. PubMed
  12. Ivanova EL, et al. 2022. Front Oncol. 12:969787. PubMed
  13. Costa B, et al. 2021. Cancers (Basel). 13:00. PubMed
  14. Moyon S, et al. 2021. Nature Communications. 12(1):3359. PubMed
  15. Marcelo A, et al. 2021. Cell Death Dis. 12:1117. PubMed
  16. Wang YJ, et al. 2021. Sci Rep. 11:8054. PubMed
  17. Chompre G, et al. 2022. Heliyon. 8:e11194. PubMed
  18. Murenu E, et al. 2021. Int J Mol Sci. 22:. PubMed
  19. Costa B, et al. 2019. Blood Adv. 3:1092. PubMed
RRID
AB_2109791 (BioLegend Cat. No. 644701)
AB_2109791 (BioLegend Cat. No. 644702)

Antigen Details

Structure
Around 50 kD
Distribution

Only expressed in astrocytes in the central nervous system.

Function
Together with other proteins to form intermediate filaments which supports astroglial cells. Astroglial cells support and nourish cells in the brain and spinal cord. If brain cells are injured through trauma or disease, astroglial cells react by rapidly prodcing more GFAP protein.
Cell Type
Astrocytes
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
2670 View all products for this Gene ID
UniProt
View information about GFAP on UniProt.org

Other Formats

View All GFAP Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-GFAP 2E1.E9 WB,ICC,IHC-F,IHC-P,FC
Alexa Fluor® 488 anti-GFAP 2E1.E9 IHC-F,ICC,IHC-P,SB
Alexa Fluor® 647 anti-GFAP 2E1.E9 IHC-F,IHC-P,SB
Alexa Fluor® 594 anti-GFAP 2E1.E9 IHC-F,IHC-P,SB
Brilliant Violet 421™ anti-GFAP 2E1.E9 IHC-F,ICC,IHC-P
Go To Top Version: 6    Revision Date: 09.25.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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