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Purified anti-Presenilin 2 Antibody

Pricing & Availability
Clone
PS2 (See other available formats)
Regulatory Status
RUO
Other Names
Presenilin-2, AD5, E5-1, PS-2, AD3LP, STM-2, gamma-secretase, PSEN2, PS2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
PS2_PURE_Presenilin2_Antibody_WB_030617
Western blot of anti-Presenilin 2 antibody (clone PS2). Lane 1: Molecular weight marker; Lane 2: SH-SY5Y Nuclear Fraction. The blot was incubated with the primary antibody at 1µg/ml overnight at 4°C, followed by incubation with a goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection.
  • PS2_PURE_Presenilin2_Antibody_WB_030617
    Western blot of anti-Presenilin 2 antibody (clone PS2). Lane 1: Molecular weight marker; Lane 2: SH-SY5Y Nuclear Fraction. The blot was incubated with the primary antibody at 1µg/ml overnight at 4°C, followed by incubation with a goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection.
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814203 25 µg 112 CHF
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814204 100 µg 276 CHF
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Description

Mutations in the genes for presenilin 1 (PS1) and 2 (PS2) are the causative factors for ~50% of early onset familial Alzheimer's disease. PS1 and PS2 are homologous transmembrane proteins, and along with nicastrin, form high molecular mass multimeric protein complexes involved in the intramembranous proteolysis of several proteins including amyloid precursor protein (APP) and Notch receptors. Absence of PS1 is associated with dramatic reductions in the levels of mature glycosylated nicastrin. In contrast, absence of PS2 is associated with only modest reductions in the levels of immature nicastrin.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised using a GST-fusion containing the complete N-terminal fragment of human Presenilin 2 (residues 1-87).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Cell type: SH-SY5Y neuroblastoma cells. Presenilin 2 is proteolytically cleaved to generate two polypeptides. The full-length PS2 protein (~55 kD) is cleaved to a ~ 35 kD N-terminal fragment and ~20 kD C-terminal fragment. The predominant species observed in cultured mammalian cells and in the brain is the processed fragment of ~35 kD. This antibody recognizes the full length and the ~35 kD cleavage fragment.


Primary antibody incubation period for WB: overnight at 4°C .

RRID
AB_2650835 (BioLegend Cat. No. 814203)
AB_2650835 (BioLegend Cat. No. 814204)

Antigen Details

Structure
Presenilin 2 is a 448 amino acid protein with a molecular mass of ~ 55 kD.
Distribution

Tissue distribution: Presenilin 2 is ubiquitously expressed in humans. In the central nervous system (CNS), it is expressed by almost all cell types including astrocytes, neurons, microglia, unmyelinating and myelinating oligodendrocytes and progenitor cells.

Cellular distribution: Cytoskeleton, endoplasmic reticulum, nucleus and plasma membrane.

Function
Presenilin 2 helps process proteins that transmit chemical signals from the cell membrane into the nucleus. Once in the nucleus, these signals turn on (activate) genes that are important for cell growth and maturation. PS2 is best known for its role in processing amyloid precursor protein, which is found in the brain and other tissues. It has been suggested that presenilin 2 works together with other enzymes to cut amyloid precursor protein into smaller segments (peptides). One of these peptides is called soluble amyloid precursor protein (sAPP), and another is called amyloid beta peptide. sAPP has growth-promoting properties and may play a role in the formation of neurons in the brain both before and after birth.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Secretases
Antigen References

1. Brunello L, et al. 2009. J. Cell Mol. Med. 13:3358. (WB) PubMed
2. Hutton M, Hardy J. 1997. Hum. Mol. Genet. 6:1639.

Gene ID
5664 View all products for this Gene ID
UniProt
View information about Presenilin 2 on UniProt.org
Go To Top Version: 2    Revision Date: 12.03.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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