Loading...

Blog Article

Within a tumor microenvironment, immunosuppressive Tregs can prevent T effector cells from properly detecting and destroying tumor antigens. Liu et al. began to investigate the mechanism Tregs are using for this suppression, finding indications that the Tregs became a sink for glucose and the resulting withdrawal caused effector/naive T cells to become senescent. The loss of glucose triggered an ATM-associated DNA damage response. They also found that MAPK, ERK1/2, p38, and STAT1/STAT3 signaling led to increased expression of p53, p21, and p16, which arrested the cell cycle and caused senescence. These studies help to define human Treg mechanisms of suppression and have important implications in immunotherapies and cancer treatments.

BioLegend provides several sensitive antibodies for the detection of phosphorylated proteins involved in these signaling pathways along with True-Phos™ Perm Buffer, which is specially designed for the detection of phosphorylated protein targets in intracellular flow cytometry staining.
Adapted from Liu, X. et al. 2018. Nat. Commun. 9:249. Pubmed
Specificity Clone
BTK Phospho (Tyr223) A16128B
BTK Phospho (Tyr223)/ITK Phospho (Tyr180) A16128C
BTK Phospho (Tyr551)/ITK Phospho (Tyr512) A16064A
ERK1/2 Phospho (Thr202/Tyr204) 6B8B69
p38 MAPK Phospho (Thr180/Tyr182) Poly29035
STAT3 Phospho (Tyr705) 13A3-1
STAT3 Phospho (Ser727) A16089B
STAT6 Phospho (Tyr641) A15137A
Related Products
True-Phos™ Perm Buffer Chk2 p38 MAPK
Western-Ready™ ECL Substrate Kit H2A.X Phospho
(Ser139) 		p53
Direct-Blot™ HRP Conjugated Antibodies p16 (CDKN2A) STAT1
ATM Phospho (Ser1981) p21 (CDKN1A) STAT3
Need help with travel funding to get to your conference? Each month, we provide a $500 travel award to alleviate the costs of attending a conference. No purchase is required, and US/Canada post-docs, junior faculty, and graduate students are eligible! Apply here...
After a brief hiatus, the BioLegend Blog returns with a renewed focus on troubleshooting help and suggestions. Check out the blog here, and then listen to our podcast, which has just passed 100,000 downloads and listens!
*Any references to promotions on this page may not be valid at this time. View our promotions page for the most up-to-date promotions.
Related Pages
ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account