Staining intracellular targets by flow cytometry can be difficult and may require specific protocols depending on your targets of interest. We've created a number of resources to better help you choose the right intracellular flow staining protocol to remove the guesswork and help you generate clear and consistent results. For staining phosphorylated intracellular targets, we've released a video protocol outlining how to stain cells using our True-Phos™ permeabilization buffer.

 
Fixation Buffers
Fixation Buffer
RBC Lysis/Fixation Solution (10X)
Permeabilization Buffers
Intracellular Staining Permeabilization Wash Buffer (10X)
True-Phos™ Perm Buffer
Buffer Sets
True-Nuclear™ Transcription Factor Buffer Set
View our Technical Protocols for Intracellular Flow Cytometry
Specificity Clone Applications
Neurofilament H & M, Phosphorylated SMI 310 IHC-P, WB
Neurofilament H, Phosphorylated SMI 31 IHC-P
RPS6 Phospho (Ser235/236) A17020B ICFC, ICC, WB
STAT1 Phospho (Tyr701) A17012A ICFC, ICC, WB, ChIP
STAT1 Phospho (Ser727) A15158B ICFC, ICC, WB, ChIP
STAT3 Phospho (Ser727) A16089B ICFC, WB, ChIP
Need a control for your phospho flow cytometry staining? Our Veri-Cells™ Phospho PBMCs can be used as a positive and negative control for staining members of the MAPK/ERK pathway. Learn more about how Veri-Cells™ can provide you the perfect control for your samples on our blog.
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