- Clone
- OX-7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Thy-1, Thy-1.1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD90, also known as Thy-1, is a 28-30 kD GPI-linked membrane glycoprotein. It is a member of the immunoglobulin superfamily and has been shown to interact with CD45 in signal transduction during lymphocyte proliferation and differentiation. CD90 is expressed on hematopoietic stem cells, neurons, thymocytes, peripheral T cells, fibroblasts, stromal cells.
Product DetailsProduct Details
- Verified Reactivity
- Rat, Mouse
- Reported Reactivity
- Rabbit, Guinea Pig
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Rat thymocyte Thy-1 antigen
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
The OX-7 antibody reacts with rat CD90 and mouse CD90.1 (Thy-1.1) (which is expressed by mouse strains of AKR/J, PL, and FVB/N), but not mouse CD90.2.
Additional reported applications (for the relevant formats) include: immunohistochemical7 and immunofluorescent8 staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, immunoprecipitation1, Western blotting1, in vitro activation of leukocytes2, induction of endothelial cell permeability3, induction of apoptosis in glomerular mesangial cells, and induction of glomerulonephritis in vivo4. -
Application References
(PubMed link indicates BioLegend citation) -
- Jeng CJ, et al. 1998. J. Cell Biol. 140:685. (IP, WB)
- Nakashima I, et al. 1991. J. Immunol. 147:1153.
- Ishizu A, et al. 1995. Int. Immunol. 7:1939.
- Eitner F. 1997. Kidney. Int. 51:69.
- Kawachi H, et al. 1992. Clin. Exp. Immunol. 88:399. (WB)
- Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
- Daniel C, et al. 2012. Lab Invest. 92:812. (IHC-P)
- Li B, et al. 2006. Kidney Int. 69:323. (ICC)
- Uchimura H, et al. 2005. J Am Soc Nephrol. 16(4):997-1004. (IHC-F)
- Inagi R, et al. 2008. J Am Soc Nephrol. 19(5):915-22. (IHC-P)
- Product Citations
-
- RRID
-
AB_2562643 (BioLegend Cat. No. 202535)
Antigen Details
- Structure
- Ig superfamily, GPI-anchored membrane glycoprotein, 28-30 kD
- Distribution
-
Hematopoietic stem cells, thymocytes, a subset of peripheral blood T cells, early myeloid and erythroid progenitors, immature B cells, neurons, activated endothelium, mast cells, dendritic cells, glomeruli
- Function
- Adhesion, signal transduction, lymphocyte co-stimulation, proliferation and differentiation of hematopoietic stem cells
- Ligand/Receptor
- Interacts with CD45
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Hematopoietic stem and progenitors, Mast cells, Neurons, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Campbell DG, et al. 1981. Biochem. J. 195:15.
2. Hosseinzadeh H, et al. 1993. J. Immunol. 150:1670. - Gene ID
- 21838 View all products for this Gene ID 24832 View all products for this Gene ID
- UniProt
- View information about CD90/CD90.1 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE/Cyanine7 anti-rat CD90/mouse CD90.1 (Thy1.1)
LOU rat thymocytes stained with OX-7 PE/Cyanine7 -
Purified anti-rat CD90/mouse CD90.1 (Thy1.1)
LOU rat thymocytes stained with purified OX-7, followed by a... FVB/N mouse frozen thymus section was fixed with 4% paraform... -
FITC anti-rat CD90/mouse CD90.1 (Thy1.1)
Lou rat thymocytes stained with OX-7 FITC -
Alexa Fluor® 488 anti-rat CD90/mouse CD90.1 (Thy1.1)
Rat thymocytes stained with OX-7 Alexa Fluor® 488 FVB/N mouse frozen thymus section was fixed with 4% paraform... -
Alexa Fluor® 647 anti-rat CD90/mouse CD90.1 (Thy1.1)
LOU rat thymocytes stained with OX-7 Alexa Fluor® 647 FVB/N mouse frozen thymus section was fixed with 4% paraform... -
PerCP/Cyanine5.5 anti-rat CD90/mouse CD90.1 (Thy1.1)
Lewis rat thymocytes were stained with CD3 APC and CD90/mous... -
APC/Cyanine7 anti-rat CD90/mouse CD90.1 (Thy1.1)
LOU rat splenocytes stained with OX-7 APC/Cyanine7 LOU rat thymocytes stained with OX-7 APC/Cyanine7 -
Pacific Blue™ anti-rat CD90/mouse CD90.1 (Thy1.1)
LOU rat thymocytes stained with OX-7 Pacific Blue™ -
PerCP anti-rat CD90/mouse CD90.1 (Thy1.1)
Lou rat thymocytes stained with OX-7 PerCP -
PE anti-rat CD90/mouse CD90.1 (Thy1.1)
LOU rat thymocytes stained with OX-7 PE -
APC anti-rat CD90/mouse CD90.1 (Thy1.1)
Lou rat thymocytes stained with OX-7 APC -
Alexa Fluor® 700 anti-rat CD90/mouse CD90.1 (Thy1.1)
LOU rat thymocytes stained with OX-7 Alexa Fluor® 700 -
Biotin anti-rat CD90/mouse CD90.1 (Thy1.1)
LOU rat thymocytes stained with OX-7 biotin, followed by Sav... -
Brilliant Violet 421™ anti-rat CD90/mouse CD90.1 (Thy1.1)
FVB mouse splenocytes were stained with CD90.1 (clone OX-7) ... C57BL/6 mouse splenocytes were stained with CD90.1 (clone OX... -
Brilliant Violet 650™ anti-rat CD90/mouse CD90.1 (Thy1.1)
Lou rat thymocytes were stained with CD90.1 (clone OX-7) Bri... -
Brilliant Violet 510™ anti-rat CD90/mouse CD90.1 (Thy1.1)
FVB mouse splenocytes were stained with CD90.1 (clone OX-7) ... Lewis rat thymocytes were stained with CD90.1 (clone OX-7) B... -
Brilliant Violet 605™ anti-rat CD90/mouse CD90.1 (Thy1.1)
FVB/N mouse splenocytes were stained with CD90.1 (clone OX-7... Lewis rat thymocytes were stained with CD90.1 (clone OX-7) B... -
Brilliant Violet 711™ anti-rat CD90/mouse CD90.1 (Thy1.1)
FVB/N mouse splenocytes were stained with CD90.1 (clone OX-7... Lewis rat thymocytes were stained with CD90.1 (clone OX-7) B... -
PE/Dazzle™ 594 anti-rat CD90/mouse CD90.1 (Thy1.1)
FVB mouse splenocytes were stained with CD90.1 (clone OX-7) ... -
APC/Fire™ 750 anti-rat CD90/mouse CD90.1 (Thy1.1)
Lewis rat thymocytes were stained with APC/Fire™ 750 anti-ra... -
Alexa Fluor® 594 anti-rat CD90/mouse CD90.1 (Thy1.1)
FVB/N mouse frozen thymus section was fixed with 4% paraform... -
TotalSeq™-A0380 anti-rat CD90/mouse CD90.1 (Thy1.1)
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TotalSeq™-B0380 anti-rat CD90/mouse CD90.1 (Thy1.1)
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TotalSeq™-C0380 anti-rat CD90/mouse CD90.1 (Thy1.1)
-
Brilliant Violet 785™ anti-rat CD90/mouse CD90.1 (Thy1.1)
Lewis rat thymocytes were stained with anti-rat CD3 FITC and...
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