Western blot of HRP anti-Syntaxin antibody (clone SP8). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 0.02 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
Western blot of HRP anti-Syntaxin antibody (clone SP8). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 0.02 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
IHC staining of HRP anti-Syntaxin antibody (clone SP8) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
IHC staining of HRP anti-Syntaxin antibody (clone SP8) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
IHC staining of HRP anti-Syntaxin antibody (clone SP8) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Syntaxin, also known as syntaxin-1A and neuron-specific antigen HPC-1, is part of the Q-SNARE protein family that participates in exocytosis. Syntaxins have a SNARE domain, an N-terminal regulatory domain, and a single C-terminal transmembrane domain. Syntaxin drives the spontaneous calcium-independent fusion of synaptic vesicles that contain v-SNARE proteins, and may form part of the fusion pore of exocytosis.
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.02 - 0.1 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes
This antibody is effective in immunohistochemistry (IHC-P) and western blotting (WB).
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