- Clone
- SM-M5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MYH11, smooth muscle Myosin heavy chain 11, SMMHC
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Save |
---|---|---|---|
673351 | 25 µg | ¥31,900 | |
673352 | 100 µg | ¥89,100 |
Myosin Heavy Chain 11, also known as MYH11, is a key protein involved in the contractile function of smooth muscle cells. This protein is a major component of the myosin II complex, which interacts with actin filaments to facilitate muscle contraction. By utilizing ATP, MYH11 enables the sliding of actin filaments, generating the mechanical force necessary for smooth muscle contraction. This function is crucial for maintaining the tone and proper functioning of various smooth muscle tissues, including those in the blood vessels, gastrointestinal tract, and respiratory system. In addition to its role in muscle contraction, MYH11 is also important for the structural integrity of smooth muscle cells. Mutations in the MYH11 gene can lead to serious medical conditions, such as familial thoracic aortic aneurysms and dissections (TAAD), where the weakening of the aortic wall poses a significant risk.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Crude human uterus lysate preparation
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).
For Western blot (WB), this antibody has significantly higher signal when detecting mouse Myosin heavy chain 11, as compared to human.
This antibody is not recommended for use in immunocytochemistry (ICC), or intracellular flow cytometry (ICFC) due to dim signal.
Antigen Details
- Structure
- Myosin heavy chain 11 is a 1,972 amino acid protein with a predicted molecular weight of 227 kD.
- Distribution
-
Cytoskeletal, cytoplasmic
- Function
- Cytoskeleton, contractile protein via ATP hydrolysis
- Interaction
- Myosin is a hexameric protein consisting of 2 heavy chains, 2 light chains, and 2 regulatory light chain subunits.
- Biology Area
- Cardiovascular Biology, Cell Motility/Cytoskeleton/Structure
- Antigen References
-
- Touyz RM, et al. 2018. Cardiovasc Res. 114:529-39.
- Babu GJ, et al. 2000. Microsc Res Tech. 50:532-40.
- Finsterer J. 2024. Forensic Sci Med Pathol. 20:756-7.
- Gene ID
- 4629 View all products for this Gene ID
- UniProt
- View information about Myosin heavy chain 11 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Myosin heavy chain 11 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Myosin heavy chain 11 | SM-M5 | IHC-P,WB |
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