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Purified anti-BAK1 Antibody

Pricing & Availability
Clone
W23097D (See other available formats)
Regulatory Status
RUO
Other Names
BCL2 antagonist/killer 1, BCL2L7, BAK, CDN1, Apoptosis regulator BAK, Bcl-2 homologous antagonist/killer
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a. W23097D_PURE_BAK1_WB_020625
Whole cell lysates (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-BAK1 (clone W23097D). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG antibody (Cat. No. 405405). Direct-Blot™ HRP anti-β-Actin antibody (Cat. No. 664803) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • a. W23097D_PURE_BAK1_WB_020625
    Whole cell lysates (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-BAK1 (clone W23097D). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG antibody (Cat. No. 405405). Direct-Blot™ HRP anti-β-Actin antibody (Cat. No. 664803) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • b. W23097D_PURE_Ab_IHC-P_020625
    HEK293 cells transfected with non-targeting siRNA (positive control, top panels) (Horizon Cat. No. D-001810-10) or with BAK1 siRNA (low expression control, bottom panels) (Horizon Cat. No. L-003305-00) using DharmaFECT 1 Transfection Reagent (Horizon Cat.No. T-2001). Cells were fixed with 4% PFA Fixation Buffer (Cat. No. 420801), permeabilized with ice cold methanol and then blocked with 5% FBS. Cells were stained with Purified anti-BAK1 (clone W23097D) (right panels) or Rat IgG2a, κ Isotype Control (Cat. No. 400502) (left panels) followed by Alexa Fluor® 647 Goat anti-rat (Cat. No. 405416). Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm.
  • c. W23097D_Purified_AB_020625
    IHC staining with anti-BAK1 (clone W23097D) was performed on formalin-fixed paraffin embedded human skin tissue following antigen retrieval using Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). The tissue sections were incubated with Purified anti-BAK1 (clone W23097D) (panel B and C) followed by Alexa Fluor® 647 Goat anti-rat (Cat. No. 405416) or secondary antibody only (panel A). Nuclei were counterstained with DAPI (Cat. No. 422801) (panel A and merged in panel C). Scale Bar: 50 µm
  • W23097D_PURE_Ab_ICFC_020625
    HEK293 cells were transfected with non-targeting siRNA (positive control, filled histogram) (Horizon Cat. No. D-001810-10) or with BAK1 siRNA (low expression control, open histogram) (Horizon Cat. No. L-003305-00) using DharmaFECT 1 Transfection Reagent (Horizon Cat.No. T-2001). Cells were fixed and permeabilized using True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Purified anti-BAK1 (clone W23097D) or Purified rat IgG2a, κ Isotype Control Antibody (open histogram, dashed line) (Cat. No. 400502) followed by Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416).
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693151 25 µg 132€
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693152 100 µg 340€
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Description

BAK1 (Bcl-2 homologous antagonist/killer) is a crucial protein involved in regulating apoptosis, or programmed cell death, which is essential for maintaining cellular homeostasis. It functions as a pro-apoptotic factor, promoting cell death in response to various stress signals. BAK1 belongs to the BCL-2 family of proteins and plays a crucial role in the mitochondrial apoptotic process by promoting mitochondrial outer membrane permeabilization, which leads to the release of apoptogenic factors into the cytosol. BAK1 is regulated through interactions with other proteins, such as BCL-2 family members, which can either inhibit or promote its apoptotic activity. Dysregulation of BAK1 has been implicated in several diseases, thus, overexpression of BAK1 can contribute to neurodegenerative and autoimmune diseases by promoting excessive cell death. Conversely, inhibition of BAK1 is associated with the development of cancers, as it allows cells to evade apoptosis and continue proliferating.

Product Details
Quality Statement

This product was carefully developed for performance and specificity using the following products from Horizon Discovery, a Revvity company:

ON-TARGETplus Non-targeting Control Pool (Horizon Cat. No. D-001810-10-50)
ON-TARGETplus siRNA BAK1 SMARTPool (Horizon Cat. No. L-003305-00-0050) 
DharmaFECT 1 Transfection Reagent (Horizon Cat. No. T-2001-01)

Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Human BAK1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 2.5 - 10.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use of this antibody in Immunocytochemistry (ICC), we recommend fixation/permeabilization using PFA-Methanol or Methanol only. We do not recommend PFA-Triton X-100 fixation/permeabilization. 

For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

Antigen Details

Structure
BAK1 protein consists of 211 amino acids with a predicted molecular weight of 23 kDa.
Distribution

Mitochondrial outer membrane

Function
• Pro-apoptotic role: BAK1 promotes apoptosis by facilitating mitochondrial outer membrane permeabilization.
• Interaction with BCL-2 family: It interacts with other BCL-2 family proteins to regulate apoptosis.
• Release of apoptogenic factors: Upon activation, BAK1 helps release factors from the mitochondria that trigger the apoptotic cascade.
Interaction
• BCL-2 and BCL-XL: These anti-apoptotic proteins bind to BAK1, inhibiting its pro-apoptotic activity.
• BAX: BAK1 forms heterooligomers with BAX, another pro-apoptotic protein, to promote mitochondrial outer membrane permeabilization.
• p53: The tumor suppressor protein p53 interacts with BAK1 in response to cellular stress, enhancing its apoptotic function.
• MCL1: This anti-apoptotic protein also interacts with BAK1, regulating its activity.
• VDAC2: BAK1 interacts with the mitochondrial voltage-dependent anion channel 2, which is involved in the release of apoptogenic factors
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Mitochondrial Function, Neurodegeneration
Molecular Family
Mitochondrial Markers
Antigen References
  1. Dadsena S, et al. 2021. Biochem Biophys Acta Biomembr. 1863:183716
  2. Shalaby R, et al. 2020. Biomolecules. 10:1638
  3. Ramesh P, et al. 2020. Apoptosis. 25:305
  4. Pena-Blanco A, et al. 2018. FEBS J. 285:416
Regulation
• Protein interactions: BAK1 is regulated through its interactions with pro- and anti-apoptotic BCL-2 family members.
• Activation by stress signals: It remains inactive until activated by various cellular stress signals.
• Post-translational modifications: Phosphorylation and other modifications can influence BAK1's activity and stability.
Gene ID
578 View all products for this Gene ID
UniProt
View information about BAK1 on UniProt.org

Other Formats

View All BAK1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-BAK1 W23097D WB,IHC-P,ICC,ICFC
Go To Top Version: 1    Revision Date: 02/06/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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