Selecting the Right Buffers for Flow Cytometry

Beyond antibody reagents, flow cytometry requires the right types of buffers for optimal staining. This convenient list separates out flow cytometry applications by their intended target. It also indicates which buffers are best-suited to your task for surface or intracellular staining and the protocols necessary for each.

Additionally, our Tandem Stabilizer helps to reduce tandem dye de-coupling when added as a supplement to our commonly used buffers for storing stained, fixed cells, as well as those used for fixation and permeabilization procedures. 

Equivalent Products

Prevent Non-Specific Brilliant™ Polymer Binding

True-Stain™ Multi-Fluor Buffer helps prevent interactions between fluorescent reagents when staining cells with multiple Brilliant Violet™ or Brilliant Ultraviolet™ antibody conjugates without reducing their signal. This alternative to BD Horizon™ Brilliant Stain Buffer can help minimize the non-specific interaction of Brilliant polymer dyes, reducing staining artifacts which may affect data interpretation. Consult our technical support team to learn about panel selection and what best practices can be used to further reduce the possibility of non-specific polymer staining. True-Stain Multi-Fluor Buffer is optimized for use with BioLegend protocols and flow cytometry reagents, including fixation and permeabilization reagents and BioLegend compensation beads. Increase confidence in your results with our full array of products.

Eliminate Monocyte Non-Specific Antibody Binding in Flow Cytometry

Monocytes and macrophages can exhibit non-specific binding of antibodies and fluorophores used in cell surface staining of live cells. BioLegend has now formulated an effective blocking reagent, True-Stain Monocyte Blocker™. This non-antibody based blocking solution has been shown to reduce non-specific monocyte binding due to the fluorophore and does not affect the desirable specific staining of monocytes. Learn more about how True-Stain Monocyte Blocker™ can help you immuno-label monocytes with confidence.

Buffers to Support your Western Blotting Experiments

Our Western-Ready™ buffers are easily integrated into your workflow to support protein extraction, gel loading and running, and detection. Explore our Western blot page to learn more about our buffers, protocols and troubleshooting tips!