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APC anti-human HLA-A2 Antibody

Pricing & Availability
Clone
BB7.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC-class I
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
BB7.2_APC_HLA-A2_Antibody_FC_1_092812
Peripheral blood lymphocytes from HLA-A2 positive (top) and HLA-A2 negative (bottom) donors were stained with anti-human HLA-A2 (clone BB7.2) APC (filled histograms) or mouse IgG2b APC isotype control (open histograms).
  • BB7.2_APC_HLA-A2_Antibody_FC_1_092812
    Peripheral blood lymphocytes from HLA-A2 positive (top) and HLA-A2 negative (bottom) donors were stained with anti-human HLA-A2 (clone BB7.2) APC (filled histograms) or mouse IgG2b APC isotype control (open histograms).
  • BB7.2_APC_HLA-A2_Antibody_FC_2_092812
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343307 25 tests 100€
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343308 100 tests 261€
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Description

HLA-A2 is most common in Northern Asia and North America populations. MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+ T cells, playing an important role in cell-mediated immune responses and tumor surveillance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Papain solubilized HLA-A2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The BB7.2 antibody recognizes human leukocyte antigen (HLA) A2 which is a subset of MHC-class I molecules encoded by A*02 alleles.

Additional reported applications (for the relevant formats) include: immunoprecipitation3.

Application References

(PubMed link indicates BioLegend citation)
  1. Brodsky FM, et al. 1979. Immunol. Rev. 47:3.
  2. Parham P and Brodsky FM. et al. 1981. Hum. Immunol. 3:277.
  3. Lubben NB, et al. 2007. Mol Biol Cell. 18:3351. (IP)
Product Citations
  1. Emert-Sedlak LA, et al. 2022. ACS Infect Dis. 8:91. PubMed
  2. Eri T, et al. 2017. Sci Rep. 46037:7. PubMed
  3. Zheng R, et al. 2023. Front Immunol. 1258156:14. PubMed
  4. Hsiue EH, et al. 2021. Science. 371:. PubMed
  5. Almshayakhchi R, et al. 2021. Front Oncol. 11:636977. PubMed
  6. Bradley S, et al. 2015. Cancer Immunol Res. 3:602. PubMed
  7. Jackson KR, et al. 2022. PNAS Nexus. 1:pgac124. PubMed
  8. Brohl AS, et al. 2021. Cell Rep. 37:110047. PubMed
  9. Lanz AL, et al. 2021. Cell Reports. 36(2):109375. PubMed
  10. Larsen BM, et al. 2021. Cell Reports. 36(4):109429. PubMed
RRID
AB_2561566 (BioLegend Cat. No. 343307)
AB_2561566 (BioLegend Cat. No. 343308)

Antigen Details

Structure
Ig superfamily
Distribution

A*02 individuals leukocytes

Function
Antigen presentation
Ligand/Receptor
CD8, CD3/TCR
Cell Type
Leukocytes
Biology Area
Immunology
Molecular Family
MHC Antigens
Gene ID
3105 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
HLA-A2
Specificity Alt (DOES NOT SHOW ON TDS):
HLA-A2
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about HLA-A2 on UniProt.org
Go To Top Version: 2    Revision Date: 02.15.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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