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GoInVivo™ Purified anti-mouse CD274 (B7-H1, PD-L1) Antibody

Pricing & Availability
Clone
10F.9G2 (See other available formats)
Regulatory Status
RUO
Other Names
B7-H1, PD-L1
Isotype
Rat IgG2b, κ
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Product Citations
publications
10F.9G2_GoInVivoPurified_CD274_B7H1_PDL1_Antibody_FC_010716
Anti-mouse PD-L1 inhibits the binding of PD-1 to immobilized PD-L1. Immobilized recombinant PD-L1-Fc chimera (coated at 1 µg/mL) was pre-incubated with increasing concentrations of anti-mouse PD-L1 (clone 10F.9G2, purple circles) or isotype control (clone RTK4530, purple squares), followed by incubation with a fixed concentration of PD-1-Fc chimera (1 µg/mL). Clone 10F.9G2 inhibits PD-1/PD-L1 interaction in a dose dependent manner.
  • 10F.9G2_GoInVivoPurified_CD274_B7H1_PDL1_Antibody_FC_010716
    Anti-mouse PD-L1 inhibits the binding of PD-1 to immobilized PD-L1. Immobilized recombinant PD-L1-Fc chimera (coated at 1 µg/mL) was pre-incubated with increasing concentrations of anti-mouse PD-L1 (clone 10F.9G2, purple circles) or isotype control (clone RTK4530, purple squares), followed by incubation with a fixed concentration of PD-1-Fc chimera (1 µg/mL). Clone 10F.9G2 inhibits PD-1/PD-L1 interaction in a dose dependent manner.
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124327 1 g 22880€
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124325 100 mg 4268€
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124329 25 mg 1892€
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124326 500 mg 14740€
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124330 50 mg 2860€
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124328 5 mg 616€
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Description

CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 1.0 EU/mg of the protein (< 0.1 pg/µg of the protein) as determined by the LAL test.
Preparation
The GoInVivo™ antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment.

Application Notes

GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage. 

Additional reported applications (for the relevant formats) include: immunofluorescence4 and blocking6,7,8,9, and immunohistochemistry of acetone-fixed frozen sections11, 12.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Maier H, et al. 2007. J. Immunol. 178:2714.
  2. Meng Q, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:4444. PubMed
  3. Scarlett UK, et al. 2012. J Exp Med. 209:495. PubMed
  4. Grabie N, et al. 2007. Circulation 116:2062. (IF)
  5. Paterson AM, et al. 2011. J. Immunol. 187:1097.
  6. Channappanavar R, et al. 2012. PLoS One 7:e39757. (Block)
  7. Schreiber HA, et al. 2010. PLoS One 5:e11453. (Block) PubMed
  8. Muthumani K, et al. 2011. J. Immunol. 187:2932. (Block) PubMed
  9. Cripps JG, et al. 2010. Hepatology 52:1350. (Block) PubMed
  10. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  11. Riella LV, et al. 2011. Am. J. Transplant 11:832-40. (IHC)
  12. Grabie N, et al. 2007. Circulation 116:2062-71. (IHC)
  13. Lei GS, et al. 2015. Infect Immun. 83:572. PubMed
Product Citations
  1. Chen X, et al. 2022. J Extracell Vesicles. 11:e12279. PubMed
  2. Bazan-Peregrino M, et al. 2021. J Immunother Cancer. 9:. PubMed
  3. Chen H, et al. 2022. J Immunother Cancer. 10:. PubMed
  4. Li D, et al. 2021. Mol Cancer Ther. 20:1926. PubMed
  5. Li H, et al. 2021. Nat Commun. 12:2773. PubMed
  6. Jaynes JM, et al. 2020. Sci Transl Med. 12:00. PubMed
  7. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
  8. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  9. Wang C, et al. 2018. Sci Transl Med. 10. PubMed
  10. Yamazaki K, et al. 2022. Sci Rep. 12:3242. PubMed
RRID
AB_2566281 (BioLegend Cat. No. 124327)
AB_2566281 (BioLegend Cat. No. 124325)
AB_2566281 (BioLegend Cat. No. 124329)
AB_2566281 (BioLegend Cat. No. 124326)
AB_2566281 (BioLegend Cat. No. 124330)
AB_2566281 (BioLegend Cat. No. 124328)

Antigen Details

Structure
40 kD type I transmembrane protein member of B7 family within the immunoglobulin receptor superfamily
Distribution

T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes

Ligand/Receptor
PD-1 (PDCD1)
Cell Type
B cells, Dendritic cells, Endothelial cells, Monocytes, NK cells, T cells
Biology Area
Cancer Biomarkers, Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.
2. Dong H, et al. 1999. Nat. Med. 5:1365.
3. Freeman G, et al. 2000. J. Exp. Med. 192:1027.

Gene ID
60533 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD274
Specificity Alt (DOES NOT SHOW ON TDS):
CD274
App Abbreviation (DOES NOT SHOW ON TDS):
FC,Block
UniProt
View information about CD274 on UniProt.org
Go To Top Version: 6    Revision Date: 01.08.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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