PerCP/Cyanine5.5 anti-human CD50 ICAM-3 Antibody anti-CD50

Pricing & Availability

Clone: CBR-IC3/1 (See other available formats)
Regulatory Status: RUO
Other Names: ICAM-3
Isotype: Mouse IgG1, κ
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Product Citations: publications

CBR-IC3slash1_PerCPCy55_CD50_Antibody_021918 — Human peripheral blood lymphocytes were stained with anti-human CD50 (ICAM-3) (clone CBR-IC3/1) PerCP/Cyanine5.5 (closed histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).

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330017

25 tests

141€

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330018

100 tests

306€

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Description

CD50 is a 120-140 kD type I membrane protein also known as intercellular adhesion molecule 3 (ICAM-3). It is broadly expressed on leukocytes, endothelial cells, and Langerhans cells. CD50 is a member of the Ig superfamily. It provides costimulatory signal for T cells and regulates cell adhesion through interaction with LFA-1 (CD11a/CD18, α_L/β₂) integrin.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: SKW3 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications include: immunoprecipitation¹and blocking^1-3.
RRID: AB_2728279 (BioLegend Cat. No. 330017)
AB_2728279 (BioLegend Cat. No. 330018)

Antigen Details

Structure: Ig superfamily, type I membrane protein, 130 kD
Distribution: Thymocytes, T and B cells, monocytes, granulocytes, endothelial cells
Function: Adhesion, costimulation
Ligand/Receptor: CD11a/CD18 (LFA-1), integrin α_L/ß₂
Cell Type: B cells, Endothelial cells, Granulocytes, Monocytes, T cells, Thymocytes
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Valent P, et al. 2001. Immunol. Rev. 179:74.
Gene ID: 3385 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):: CD50
Specificity Alt (DOES NOT SHOW ON TDS):: CD50
App Abbreviation (DOES NOT SHOW ON TDS):: FC
UniProt: View information about CD50 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All CD50 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD50 (ICAM-3)	CBR-IC3/1	FC,IP
PE anti-human CD50 (ICAM-3)	CBR-IC3/1	FC
APC anti-human CD50 (ICAM-3)	CBR-IC3/1	FC
PerCP/Cyanine5.5 anti-human CD50 (ICAM-3)	CBR-IC3/1	FC
PE/Cyanine7 anti-human CD50 (ICAM-3)	CBR-IC3/1	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 02.19.2018

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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