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Purified anti-human CD5 Antibody

Pricing & Availability
Clone
UCHT2 (See other available formats)
Regulatory Status
RUO
Workshop
III 518
Other Names
Leu-1, Ly-1, T1, Tp67
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
UCHT2
Human peripheral blood lymphocytes were stained with purified CD5 (UCHT2) (solid line) or purified mouse IgG1, κ (dashed line), followed by anti-mouse IgG PE.
  • UCHT2
    Human peripheral blood lymphocytes were stained with purified CD5 (UCHT2) (solid line) or purified mouse IgG1, κ (dashed line), followed by anti-mouse IgG PE.
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300602 100 µg 79€
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Description

CD5 is a 67 kD single chain type I glycoprotein also known as Leu-1, Ly-1 and T1. It is a member of the scavenger receptor superfamily found on T cells, thymocytes, B cell subsets, chronic B lymphocytic leukemia (B-CLL), and peripheral blood dendritic cells. CD5 modulates T and B cell receptor signaling, thymocyte maturation, and T-B cell interactions upon binding to ligands such as CD72.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Capuchin Monkey, Common Marmoset, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, it is recommended to use at ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting2 and immunohistochemical staining of acetone-fixed frozen sections2,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York.
  2. Renaudineau Y, et al. 2005. Blood 106:2781. (WB IHC)
  3. Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437.
  4. Saliba AE, et al. 2010. P. Natl. Acad. Sci. USA 107:14524. PubMed
  5. Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
Product Citations
  1. Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed
  2. Xu C, et al. 2022. iScience. 25:105123. PubMed
  3. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  4. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  5. Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed
  6. Olin A, et al. 2018. Cell. 174:1277. PubMed
  7. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  8. Henrick BM, et al. 2021. Cell. . PubMed
  9. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  10. Lakshmikanth T, et al. 2020. Cell Reports. 32(3):107923. PubMed
  11. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
  12. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
  13. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
  14. Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
RRID
AB_314088 (BioLegend Cat. No. 300602)

Antigen Details

Structure
Scavenger receptor superfamily, 67 kD
Distribution

T cells, thymocytes, B cell subset, B cell CLL, peripheral blood dendritic cells

Function
Modulates TCR, BCR signaling, thymocyte maturation, T-B cell interaction
Ligand/Receptor
CD72
Cell Type
B cells, Dendritic cells, Leukemia, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Kipps T. 1988. Adv. Immunol. 47:117.
2. Resnick D, et al. 1993. Trends Biochem. Sci. 19:5.
3. Wood GS, et al. 1992. Am. J. Pathol. 14:789.

Gene ID
921 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD5
Specificity Alt (DOES NOT SHOW ON TDS):
CD5
App Abbreviation (DOES NOT SHOW ON TDS):
FC,IHC-F,WB
UniProt
View information about CD5 on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 01.19.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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