Ultra-LEAF Purified anti-human TIGIT VSTM3 Antibody anti-TIGIT

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Clone: A15153G (See other available formats)
Regulatory Status: RUO
Other Names: T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM
Isotype: Mouse IgG2a, κ
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Product Citations: publications

1_A15153G_ULEAF_TIGIT_Antibody_1_032817 — Human peripheral blood leukocytes were stained with CD3 FITC and Ultra-LEAF purified anti-human TIGIT (clone A15153G) (top) or purified mouse IgG2a, κ isotype control (bottom), followed by PE goat anti-mouse IgG. Data shown were gated on lymphocyte population.

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372719

100 µg

168€

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372720

1 mg

458€

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Description

T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as VSTM3 or WUCAM, is a 26 kD, type I transmembrane protein and is a member of the PVR (poliovirus receptor) family of immunoglobulin-like domain containing proteins. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. TIGIT is a negative regulator of NK and T cell activation. Expression of TIGIT is associated with decreased functionality of CD8 T cells in chronic viral infection and tumors. TIGIT also promotes the differentiation of tolerogenic phenotype in dendritic cells with an increased secretion of IL-10 and a diminished production of IL-12.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human TIGIT.
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone can suppress anti-CD3 induced T cell proliferation in vitro based on in-house testing.

This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).

Additional reported applications (for the relevant formats) include: Blocking¹.
Additional Product Notes: View more applications data for this product in our Scientific Poster Library.

Watch a Scientific Poster video of this product.
RRID: AB_2650966 (BioLegend Cat. No. 372719)
AB_2650966 (BioLegend Cat. No. 372720)

Antigen Details

Structure: 26kD; type I transmembrane protein, Ig-like V-type domain, ITIM motif.
Distribution: Activated T cells, Regulatory T cells (Treg), Follicular Helper T cells (TFH), NK cells.
Function: Cell signaling, negative regulation of T cells, T cell tolerance, T cell anergy.
Ligand/Receptor: CD155 (PVR), CD112 (PVRL2, NECTIN-2).
Cell Type: NK cells, T cells, Tfh, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Inhibitory Molecules, Signal Transduction
Molecular Family: Adhesion Molecules, Immune Checkpoint Receptors
Antigen References: 1. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. 106:17858.
2. Yu X, et al. 2009. Nat. Immunol. 10:48.
3. Johnston R, et al. 2014. Cancer Cell. 26:923.
Gene ID: 201633 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):: TIGIT
Specificity Alt (DOES NOT SHOW ON TDS):: TIGIT (VSTM3)
App Abbreviation (DOES NOT SHOW ON TDS):: FC
UniProt: View information about TIGIT on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

Do you guarantee that your antibodies are totally pathogen free?: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Does BioLegend test each Ultra-LEAF™ antibody by functional assay?: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?: No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All TIGIT (VSTM3) Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human TIGIT (VSTM3)	A15153G	FC,Block
APC/Fire™ 750 anti-human TIGIT (VSTM3)	A15153G	FC
APC anti-human TIGIT (VSTM3)	A15153G	FC
PE anti-human TIGIT (VSTM3)	A15153G	FC
Brilliant Violet 421™ anti-human TIGIT (VSTM3)	A15153G	FC
Brilliant Violet 605™ anti-human TIGIT (VSTM3)	A15153G	FC
PE/Dazzle™ 594 anti-human TIGIT (VSTM3)	A15153G	FC
PerCP/Cyanine5.5 anti-human TIGIT (VSTM3)	A15153G	FC
PE/Cyanine7 anti-human TIGIT (VSTM3)	A15153G	FC
Ultra-LEAF™ Purified anti-human TIGIT (VSTM3)	A15153G	FC
Biotin anti-human TIGIT (VSTM3)	A15153G	FC
Alexa Fluor® 647 anti-human TIGIT (VSTM3)	A15153G	FC
TotalSeq™-A0089 anti-human TIGIT (VSTM3)	A15153G	PG
TotalSeq™-B0089 anti-human TIGIT (VSTM3)	A15153G	PG
TotalSeq™-C0089 anti-human TIGIT (VSTM3)	A15153G	PG
KIRAVIA Blue 520™ anti-human TIGIT (VSTM3)	A15153G	FC
APC/Cyanine7 anti-human TIGIT (VSTM3)	A15153G	FC
Brilliant Violet 510™ anti-human TIGIT (VSTM3)	A15153G	FC
Brilliant Violet 785™ anti-human TIGIT (VSTM3) Antibody	A15153G	FC
TotalSeq™-D0089 anti-human TIGIT (VSTM3)	A15153G	PG
Brilliant Violet 711™ anti-human TIGIT (VSTM3)	A15153G	FC
PE/Fire™ 640 anti-human TIGIT (VSTM3)	A15153G	FC
PE/Fire™ 810 anti-human TIGIT (VSTM3)	A15153G	FC
PE/Cyanine5 anti-human TIGIT (VSTM3)	A15153G	FC
PerCP/Fire™ 806 anti-human TIGIT (VSTM3)	A15153G	FC
PerCP/Fire™ 780 anti-human TIGIT (VSTM3)	A15153G	FC
Spark Red™ 718 anti-human TIGIT (VSTM3) (Flexi-Fluor™)	A15153G	FC
PE/Fire™ 700 anti-human TIGIT (VSTM3)	A15153G	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 04.04.2017

For Research Use Only. Not for diagnostic or therapeutic use.

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