Untransfected (panel A) or multi-tag transfected (panel B) HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Cells were then intracellularly stained with 5.0 µg/mL (1:100 dilution) Alexa Fluor® 647 anti-c-Myc antibody (clone 9E10) overnight at 4°C. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
Untransfected (panel A) or multi-tag transfected (panel B) HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Cells were then intracellularly stained with 5.0 µg/mL (1:100 dilution) Alexa Fluor® 647 anti-c-Myc antibody (clone 9E10) overnight at 4°C. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
The 9E10 monoclonal antibody recognizes the amino acid sequence EQKLISEEDL, which is a specific portion of the human c-myc gene product. This antibody is highly specific and is unlikely to alter the activity of the cloned sequence.
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody. Reactivity with endogenous myc in applications other than IHC-P (ICC, WB, etc.) is not verified.
Application References
(PubMed link indicates BioLegend citation)
Rao H, et al. 2001. Nature. 410:955.
Peters CS, et al. 2001. J Biol Chem. 276:13718.
Munro S, Pelham HR. 1987. Cell. 48:899.
Evan GI, et al. 1985. Mol Cell Biol. 5:3610.
Product Citations
Merljak E, et al. 2023. Nat Commun. 14:1995. PubMed
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