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Anti-HA Tag Antibody (Previously Covance catalog# PRB-101C)

Pricing & Availability
Clone
Poly9023 (See other available formats)
Regulatory Status
RUO
Other Names
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Previously
Covance Catalog# PRB-101C
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly9023_Purified_HA-Tag_Antibody_WB_032615
Western blot analysis of HA-Tag antibody to Postitag control protein.
  • Poly9023_Purified_HA-Tag_Antibody_WB_032615
    Western blot analysis of HA-Tag antibody to Postitag control protein.
Cat # Size Price Quantity Check Availability Save
923503 25 µL 60€
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923501 200 µL 313€
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923502 500 µL 492€
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Description

Influenza Hemagglutinin (HA) is a viral membrane glycoprotein responsible for binding of the virus to the cells expressing sialic acid-containing surface receptors, such as erythrocytes and the cells in the upper respiratory tract. The hemagglutinin epitope, YPYDVPDYA, has been widely used as a tag in recombinant protein expression vectors.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Polyclonal antibody HA.11 was raised against the twelve amino acid sequence CYPYDVPDYASL.
Preparation
Serum
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

WB, IP
Recommended Usage

The optimal working dilution should be determined for each specific assay condition.
• WB: 1:1,000*
• IP: 1:150

Application Notes

This antibody is effective in immunoblotting (WB) and immunoprecipitation (IP) of tagged proteins.

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

This antibody recognizes the same hemagglutinin flu epitope YPYDVPDYA as the monoclonal antibody, HA.11 clone 16B12. This polyclonal antibody facilitates epitope tag experiments in murine systems and is useful in experiments involving two proteins labeled with two different tags.


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Barrott JJ, et al. 2011. PNAS. 108: 12752. (WB, IP)
  2. Pecot MY, Malhotra V. 2004. Cell. 116:99.
  3. Delmas C, et al. 2003. J Biol Chem. 278:12443. (IF)
  4. Caplan S, et al. 2001. J Cell Biol. 154:109. (IF, WB)
  5. Yeo C, Whitman M. 2001. Mol Cell. 7:949.
  6. Sander TL, et al. 2000. J Biol Chem. 275:12857. (WB)
  7. Bosshart H, et al. 1994. J Cell Biol. 126:1157. (IF, IP)
Product Citations
  1. Khan I, et al. 2021. Methods Mol Biol. 2262:281. PubMed
  2. Ithinji DG, et al. 2022. NPJ Vaccines. 7:166. PubMed
  3. Li W, et al. 2023. Life Sci Alliance. 6: . PubMed
  4. Wallon L, et al. 2022. Proc Natl Acad Sci U S A. 119:e2204481119. PubMed
  5. Zhu Z, et al. 2016. J Virol. 90(24):11106-11121. PubMed
  6. Tazat K, et al. 2021. Mol Biol Cell. 32:605. PubMed
  7. Hao L, et al. 2021. Cell Prolif. :e12994. PubMed
  8. Zeng J, et al. 2020. Cell Stem Cell. 27(4):618-632.e9. PubMed
  9. Villeneuve J, et al. 2017. Mol Biol Cell. 28(1):141-151. PubMed
  10. Ting Y, et al. 2017. J Biol Chem. 292(2):585-596. PubMed
  11. Khan I, et al. 2022. Cell Rep. 38:110322. PubMed
  12. Trautz B, et al. 2016. J Virol. 90(23):10915-10927. PubMed
  13. Li J, et al. 2021. Med (N Y). 0.152083333. PubMed
  14. Wang S, et al. 2022. Methods Mol Biol. 2473:181. PubMed
RRID
AB_2565437 (BioLegend Cat. No. 923501)
AB_2565437 (BioLegend Cat. No. 923502)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
Specificity (DOES NOT SHOW ON TDS):
HA Tag
Specificity Alt (DOES NOT SHOW ON TDS):
HA Tag
App Abbreviation (DOES NOT SHOW ON TDS):
WB,IP
UniProt
View information about HA Tag on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All HA Tag Reagents Request Custom Conjugation
Description Clone Applications
Anti-HA Tag Poly9023 WB,IP
Purified anti-HA.11 Epitope Tag Poly9023 WB,IP,ChIP,ICC,Purification
Go To Top Version: 6    Revision Date: 04.27.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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