APC anti-rat CD45RA Antibody anti-CD45RA

Pricing & Availability

Clone: OX-33 (See other available formats)
Regulatory Status: RUO
Other Names: Leukocyte common antigen, high molecular weight form
Isotype: Mouse IgG1, κ
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Product Citations: publications

OX-33_APC_072710 — LOU rat splenocytes stained with OX-33 APC

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202313

25 µg

76€

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202314

100 µg

176€

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Description

CD45 is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets. CD45RA is one of the CD45 isoforms with a molecular weight of 200-220 kD. It is expressed almost exclusively on B cells. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins, including galectin-1, CD2, CD3, and CD4.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Leukocyte common antigen purified from rat splenocytes.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections¹.
RRID: AB_10639733 (BioLegend Cat. No. 202313)
AB_10639733 (BioLegend Cat. No. 202314)

Antigen Details

Structure: Protein tyrosine phosphatase, type I transmembrane protein, hign molecular weight isoform approximately 200-220 kD
Distribution: Primarily on B lymphocytes
Function: B cell receptor signal transduction
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Cell Type: B cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
Molecular Family: CD Molecules
Antigen References: 1. Sunderland CA, et al. 1979. Eur. J. Immunol. 9:155.
2. Woolett GR, et al. 1985. Eur. J. Immunol. 15:168.
Gene ID: 24699 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):: CD45RA
Specificity Alt (DOES NOT SHOW ON TDS):: CD45RA
App Abbreviation (DOES NOT SHOW ON TDS):: FC
UniProt: View information about CD45RA on UniProt.org

Documentation

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Cell Surface Flow Cytometry Staining Protocol

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Pathways

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Other Formats

View All CD45RA Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-rat CD45RA	OX-33	FC,IHC-F
Biotin anti-rat CD45RA	OX-33	FC
FITC anti-rat CD45RA	OX-33	FC
PE anti-rat CD45RA	OX-33	FC
Alexa Fluor® 488 anti-rat CD45RA	OX-33	FC
APC anti-rat CD45RA	OX-33	FC
PerCP/Cyanine5.5 anti-rat CD45RA	OX-33	FC
PE/Cyanine7 anti-rat CD45RA	OX-33	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

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