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PE/Cyanine7 anti-mouse Ly-6A/E (Sca-1) Antibody

Pricing & Availability
Clone
D7 (See other available formats)
Regulatory Status
RUO
Other Names
Sca-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
D7_PECy7_091207
C57BL/6 mouse splenocytes stained with D7 PE/Cyanine7
  • D7_PECy7_091207
    C57BL/6 mouse splenocytes stained with D7 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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108113 25 µg 82€
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108114 100 µg 253€
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Description

Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent mouse T-cell line (CTL-L)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The Ly-6A/E antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed, paraffin-embedded samples9.

The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14 

Application References

(PubMed link indicates BioLegend citation)
  1. Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP)
  2. Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB)
  3. Codias EK, et al. 1990. J. Immunol. 144:2197.
  4. Malek TR, et al. 1986. J. Exp. Med. 164:709.
  5. Codias EK, et al. 1990. J. Immunol. 145:1407.
  6. Ivanov V, et al. 1994. J. Immunol. 153:2394.
  7. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  8. Fleming T, et al. 1994. J. Immunol. 153:1955.
  9. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC)
  10. Umland O, et al. 2007. J. Immunol. 178:4147.
  11. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed
  12. Pronk CJ, et al. 2011. J. Exp Med. PubMed
  13. English A, et al. 2000. J. Immunol. 165:3763. (IHC)
  14. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  15. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed
Product Citations
  1. Lang J, et al. 2020. Nat Commun. 11:1338. PubMed
  2. Hong X, et al. 2022. Cell Stem Cell. 29:1298. PubMed
  3. Pioli KT, et al. 2023. iScience. 26:106223. PubMed
  4. Bobadilla Muñoz M, et al. 2023. Front Cell Dev Biol. 11:1128534. PubMed
  5. Wu J, et al. 2022. Stem Cells Int. 2022:4249843. PubMed
  6. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  7. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  8. Moiseeva V, et al. 2023. Nature. 613:169. PubMed
  9. Huang D, et al. 2023. Exp Hematol Oncol. 12:11. PubMed
  10. Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed
  11. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  12. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  13. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  14. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
  15. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  16. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
  17. Shen Y, et al. 2021. Comput Struct Biotechnol J. 19:5360. PubMed
  18. Gutiérrez-Gutiérrez Ó, et al. 2021. EMBO Rep. 22:e52905. PubMed
  19. DellOrso S, et al. 2021. STAR Protocols. 2(2):100451. PubMed
  20. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  21. Hoffman D, et al. 2021. Immunity. 54:2712. PubMed
  22. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  23. Beauchamp EM, et al. 2021. Blood Cancer Discov. 6:. PubMed
  24. Campanario S, et al. 2021. Front Cell Dev Biol. 8:620409. PubMed
  25. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  26. Solanas G, et al. 2017. Cell. 170:678. PubMed
  27. Gross ETE, et al. 2018. Oncoimmunology. 8:e1404212. PubMed
  28. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  29. Arai F, et al. 2020. Cell Systems. 11(6):640-652.e5. PubMed
  30. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  31. Ankawa R, et al. 2021. Developmental Cell. . PubMed
  32. Kim SP, et al. 2021. Cell Reports. 36(9):109626. PubMed
  33. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  34. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  35. Barman PK, et al. 2019. J Immunol. 202:2720. PubMed
  36. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  37. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  38. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  39. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  40. Telford W, et al. 2017. Cytometry A. 91:314. PubMed
  41. Dong S, et al. 2021. Nature. 591:117. PubMed
  42. Smith E, et al. 2009. J Immunol. 183:5685. PubMed
  43. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  44. Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed
  45. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
  46. Duan Y, et al. 2018. Stem Cells. 36:1430. PubMed
  47. García-Prat L, Muñoz-Cánoves P, Martínez-Vicente 2017. Muscle Stem Cells. 10.1007/978-1-4939-6771-1_14. PubMed
  48. Zaro BW, et al. 2020. eLife. 9:00. PubMed
  49. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  50. Cho K, et al. 2021. iScience. 24:103117. PubMed
  51. Dagher T, et al. 2021. J Exp Med. 218:. PubMed
  52. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  53. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  54. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  55. Sevin M, et al. 2018. Nat Commun. 9:1431. PubMed
  56. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  57. Gough D, et al. 2014. Blood. 124:2252. PubMed
  58. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  59. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  60. Bota-Rabassedas N, et al. 2021. Cell Reports. 35(3):109009. PubMed
  61. Giraddi R, et al. 2015. Nat Commun. 6: 8487. PubMed
  62. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  63. Wang S, et al. 2021. Commun Biol. 22:. PubMed
  64. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  65. Azazmeh N, et al. 2020. Nat Commun. 2.340972222. PubMed
  66. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  67. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  68. Onai N, et al. 2020. Int J Mol Sci. :22. PubMed
  69. Kang M, et al. 2021. Front Cell Dev Biol. 8:596622. PubMed
  70. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  71. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  72. Liu F, et al. 2021. Cell Reports. 35(10):109225. PubMed
  73. Jin Y, et al. 2020. PeerJ. 8:e10374. PubMed
  74. Yosef R, et al. 2016. Nat Commun. 7: 11190. PubMed
  75. Christoforou N, et al. 2013. PLoS One. 8:65963. PubMed
  76. Zheng Z, et al. 2021. Nat Commun. 12:6202. PubMed
  77. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  78. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  79. Yoshinaga M, et al. 2022. Nat Commun. 13:6435. PubMed
  80. Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
  81. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  82. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  83. Iwanami N, et al. 2020. iScience. 23:101260. PubMed
  84. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
  85. Chavez JS, et al. 2022. Cells. 11:. PubMed
  86. Fielding C, et al. 2022. Nat Commun. 13:543. PubMed
  87. Del Barco Barrantes I, et al. 2018. Stem Cell Reports. 10:257. PubMed
  88. Kang YA, et al. 2020. J Exp Med. 217:00:00. PubMed
  89. Lerbs T, et al. 2020. JCI Insight. 5:00. PubMed
  90. Zhang B, et al. 2021. Nature. 599:471. PubMed
  91. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  92. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
  93. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
  94. Youssif C, et al. 2018. EMBO Mol Med. 10:e8403. PubMed
  95. Hacein-Bey-Abina S, et al. 2020. Exp Hematol. 88:15. PubMed
  96. Man N, et al. 2021. JCI Insight. 6:. PubMed
RRID
AB_493596 (BioLegend Cat. No. 108113)
AB_493596 (BioLegend Cat. No. 108114)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells

Function
Regulates B and T cell responses
Cell Type
B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
Ly-6A/E
Specificity Alt (DOES NOT SHOW ON TDS):
Ly-6A/E
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about Ly-6A/E on UniProt.org
Go To Top Version: 3    Revision Date: 07.11.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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