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- Clone
- 2F8B08 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Forkhead box protein O1A (FOXO1A), Forkhead in rhabdomyosarcoma (FKHR)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Total cell lysates (15 µg total protein) from HEL (negative control, Lane 1), HeLa (reduced expression control, Lane 2), and Daudi cells (Lane 3) were resolved by 4-20% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-FOXO1 Antibody, clone 2F8B08, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas. -
293T cells were stained with purified anti-FOXO1 (clone 2F8B08) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody. Nuclei were stained with DAPI (blue). -
Immunoprecipitation of FOXO1 from Hela cell extracts. Lane 1 is 8% input. Immunoprecipitation was performed using protein G resins only (lane 2), mouse IgG isotype control (lane 3), and anti-FOXO1 antibody (clone 2F8B08, lane 4). Western blot was performed using anti-FOXO1 antibody (clone 2F8B08). -
IHC staining using purified anti-FOXO1 (clone 2F8B08) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using 1X Tris-Buffered Saline (final concentration 0.05M) with Tween-20 (Cat. No. 925501), the tissue was incubated with 10 μg/mL of antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-mouse IgG antibody (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scalebar = 50 μM
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 658102 | 100 µg | 193€ | ||||
FOXO1 is a transcription factor, belonging to the O subfamily of forkhead box-containing proteins. The forkhead proteins are substrates of Akt/PKB and glucocorticoid-induced kinases that mediate insulin signaling. FOXO1 binds to the conserved insulin-responsive elements and acts as a transcriptional activator. The FOXO1 target genes are involved in cell metabolism, growth, and differentiation. Upon insulin stimulation, FOXO1 proteins are phosphorylated by AKT/PKB through the PI3K pathway, resulting in the translocation of FOXO1 proteins from the nucleus to the cytoplasm. The expression of FOXO1 target genes are then inhibited by FOXO1. FOXO1 has been reported to regulate maturation and class-switch recombination of B cells, as well as participating in regulatory T cell function. FOXO1-deficient mice are embryonic lethal, suggesting that FOXO1 has crucial functions in development which cannot be compensated by other FoxO family members.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial human FOXO1 recombinant protein (544-655aa)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IP, IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunocytochemistry, a concentration of 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 5.0 - 20 μg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 8.0 - 10. µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2562793 (BioLegend Cat. No. 658102)
Antigen Details
- Structure
- 655 amino acids, predicted molecular weight of 70 kD. Contains a C-terminal trans-activating domain and a Fork-head domain responsible for DNA binding.
- Distribution
-
Cytoplasm, Nucleus
- Function
- FOXO1 is a transcription factor mediating Insulin signaling. It regulates expression of the genes involved in cell growth, differentiation, and metabolism.
- Interaction
- FOXO1 interacts with RUNX2, NLK, SIRT1, PRMT1, SKP2, ATF4, and p300.
- Cell Type
- B cells, Tregs
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Trinh DL, et al. 2013. Blood. 121:3666.
2. Ouyang W, et al. 2012. Nature. 491:554.
3. Ferdous A, et al. 2012. Transcription. 3:221.
4. Cifarelli V, et al. 2012. Mol. Endocrinol. 26:1213.
5. Kamagate A, et al. 2008. Cell Cycle. 7:3162.
6. Kousteni S. 2012. Bone. 50:437. - Gene ID
- 2308 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- FOXO1
- Specificity Alt (DOES NOT SHOW ON TDS):
- FOXO1
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB,ICC,IP,IHC-P
- UniProt
- View information about FOXO1 on UniProt.org
Related FAQs
Other Formats
View All FOXO1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-FOXO1 | 2F8B08 | WB,ICC,IP,IHC-P |
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