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- Clone
- Poly29038 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GKPIPNPLLGLDST epitope tag, GKPIPNPLLGLDST tag, Protein Rev, Regulator of expression of viral proteins, Rev, V5 epitope tag
- Previously
-
Covance Catalog# PRB-189P
- Isotype
- Rabbit Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot analysis of cell lysates from HeLa cells transfected without or with V5-tagged plasmid using V5 Rabbit primary antibody (clone Poly 29038, 1:10000 dilution, 0.1 µg/ml) and HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807, 1:8000 dilution). was used as a loading control. -
CHO cells untransfected (negative control, panel A) and transfected (positive control, panel B) with a multi-tag construct containing the V5 epitope were fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 0.5% Triton X-100 for 10 minutes. The cells were then blocked with 5% FBS for 1 hour at room temperature. Cells were stained with 2 µg/mL of purified anti-V5 Epitope Tag (clone Poly29038), followed by incubation with Alexa Fluor® 594 goat anti-rabbit IgG (Cat. No. 406418) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 60X objective. Scale bar: 50 µm
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 903802 | 25 µL | 72€ | ||||
| 903801 | 100 µL | 265€ | ||||
The V5 epitope tag is used to label a protein of interest so that the tagged protein can be analyzed and visualized. Antibodies against V5 epitope tag recognize tagged proteins and allow for specific detection in several immunochemical assays such as western blotting, ELISAs and immunostaining techniques.
Product DetailsProduct Details
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Polyclonal V5 antisera was raised against a synthetic peptide representing amino acid residues 95 to 108 (GKPIPNPLLGLDST) of RNA polymerase alpha subunit of simian parainfluenza virus 5 conjugated to KLH.
- Formulation
-
Phosphate-buffered solution with 0.09% sodium azide.
Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service. - Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
WB - Quality tested
ICC, IHC, ELISA - Verified
Purification - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 0.2 µg/mL (1:5000 - 1:10000 dilution). For immunocytochemistry, a concentration range of 0.1 - 5.0 μg/mL is recommended. For immunohistochemistry, a dilution range of 1:100 - 400 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in immunoblotting, immunocytochemistry, and ELISA.
*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.
The antisera was purified via affinity chromatography using the peptide immobilized on solid support.
This product may contain other non-IgG subtypes.
Product detects V5 epitope tag in ICC using Fixation Buffer (Cat. No. 420801) followed by permeabilization with either 0.5% Triton-X or 100% ice-cold methanol, or with fixation/permeabilization with 100% ice-cold methanol. -
Application References
(PubMed link indicates BioLegend citation) -
- J Dixelius, et al. 2003. J. Biol. Chem. 278:40973.
- Product Citations
-
- RRID
-
AB_2565034 (BioLegend Cat. No. 903802)
AB_2565034 (BioLegend Cat. No. 903801)
Antigen Details
- Biology Area
- Cell Biology
- Gene ID
- NA
- Specificity (DOES NOT SHOW ON TDS):
- V5 Epitope Tag
- Specificity Alt (DOES NOT SHOW ON TDS):
- V5 Epitope Tag
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB,ICC,IHC,ELISA,Purification
- UniProt
- View information about V5 Epitope Tag on UniProt.org
Other Formats
View All V5 Epitope Tag Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-V5 Epitope Tag | Poly29038 | WB,ICC,IHC,ELISA,Purification |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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