Alexa Fluor 488 anti-human CD202b Tie2/Tek Antibody anti-CD202b

Pricing & Availability

Clone: 33.1 (Ab33) (See other available formats)
Regulatory Status: RUO
Other Names: Tie2, TEK
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human endothelial cell line, HUVEC, stained with 33.1 Alexa Fluor® 488

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334208

100 tests

235€

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Description

CD202b is a 145 kD type I transmembrane protein, also known as Tie2 or TEK. It is a member of the receptor tyrosine kinase (RTK) family of proteins and is expressed by endothelial cells and their progenitors, quiescent hematopoietic stem cells (HSCs), and a subpopulation of monocytes. Angiopoietin-1 (Ang-1) is an activator of CD202b to promote, maintain, and stabilize mature vessels and to maintain HSCs in quiescent state. Ang-2 is another ligand of CD202b, which is involved in postnatal angiogenesis and in antagonizing the effects of Ang-1. Tie2 binds to Ang-4 as well.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant extracellular domain of human Tie2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications include: immunoprecipitation, Wstern blot, immunohistochemical staining of frozen tissue sections and ELISA
RRID: AB_1186028 (BioLegend Cat. No. 334208)

Antigen Details

Structure: 145 kD type I transmembrane protein, receptor tyrosine kinase (RTK) family
Distribution: Endothelial cells, subsets of hematopoietic stem cells and and a subset of monocytes
Function: Angiogenesis, hemotopoiesis, monocytes chemotaxis
Ligand/Receptor: Angiopoietin-1 (Ang-1), Ang-2, and Ang-4
Cell Type: Endothelial cells, Hematopoietic stem and progenitors, Monocytes
Biology Area: Angiogenesis, Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: 1.De Palma M, et al. 2005. Cancer Cell. 8:211
2.Shaw JP, et al. 2004. Blood Cells Mol. Dis. 32:168
3. Hsu HC, et al. 2000. Blood. 96:3757
4. Arai F, et al. 2004. Cell. 118:149
Gene ID: 7010 View all products for this Gene ID
UniProt: View information about CD202b on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD202b Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD202b (Tie2/Tek)	33.1 (Ab33)	FC,IP,WB,IHC-F,ELISA
Biotin anti-human CD202b (Tie2/Tek)	33.1 (Ab33)	FC
PE anti-human CD202b (Tie2/Tek)	33.1 (Ab33)	FC
Alexa Fluor® 488 anti-human CD202b (Tie2/Tek)	33.1 (Ab33)	FC
Alexa Fluor® 647 anti-human CD202b (Tie2/Tek)	33.1 (Ab33)	FC
TotalSeq™-A0588 anti-human CD202b (Tie2/Tek)	33.1 (Ab33)	PG

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

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