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- Clone
- W22110B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Nitric oxide synthase 3 (NOS3), Endothelial Nos (eNos), constitutive NOS (cNOS)
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
EA.hy926 cells (positive cell line, filled histogram) and HeLa cells (negative cell line, open histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with Alexa Fluor® 488 Anti-NOS3 (eNOS) (clone W22110B) or Alexa Fluor® 488 Rat IgG2b, κ Isotype Control Antibody (open histogram, dashed line) (Cat No. 400625).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 626755 | 25 tests | 144€ | ||||
| 626756 | 100 tests | 360€ | ||||
Nitric oxide synthase 3 (NOS3), also commonly known as endothelial nitric oxide synthase (eNOS), is a critical enzyme primarily found in the endothelial cells lining the blood vessels. Its main function is to catalyze the formation of nitric oxide (NO) from L-arginine. NO is a signaling molecule with profound effects on vascular health and overall physiology, regulating blood vessel tone and diameter in response to various physiological cues including shear stress, neurotransmitters, and hormones. NO relaxes and dilates blood vessels, thereby promoting proper blood flow and reducing blood pressure. Additionally, NOS3-derived NO helps prevent the formation of blood clots, reduces inflammation in the blood vessel walls, and maintains vascular homeostasis, contributing significantly to cardiovascular health. Dysfunction or impaired activity of NOS3 is implicated in various cardiovascular diseases, making it an important target for therapeutic interventions aimed at preserving vascular function.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant KLH-conjugated peptide fragment of human NOS3
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Blue Laser (488 nm)
Antigen Details
- Structure
- NOS3 is a 1205 amino acid protein with a predicted molecular weight of 133.3 kD.
- Distribution
-
Cytoplasmic, vascular endothelial cells
- Function
- Enzyme, nitric oxide (NO) synthesis from L-arginine. Transcriptionally regulated by SP1, SP3, ETS-1, YY1.
- Interaction
- NOS3 is regulated via binding of its cofactor BH4, which is required to efficiently produce NO.
- Cell Type
- Endothelial cells
- Biology Area
- Cardiovascular Biology
- Molecular Family
- Enzymes and Regulators
- Antigen References
-
- Mineo C and Shaul PW. 2012. Adv Exp Med Biol. 729:51-62.
- Hong FF, et al. 2019. Inflamm Res. 68:429-441.
- Siragusa M and Fleming I. 2016. Pfulgers Arch. 468:1125-1137.
- Musicki B and Burnett AL 2006. Exp Bil Med. 231:154-65.
- Gene ID
- 4846 View all products for this Gene ID
- UniProt
- View information about NOS3 on UniProt.org
Related FAQs
Other Formats
View All NOS3 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-NOS3 (eNOS) | W22110B | IHC-P,ICC,WB,ICFC |
| Alexa Fluor® 488 anti-NOS3 (eNOS) | W22110B | ICFC |
| Alexa Fluor® 647 anti-NOS3 (eNOS) | W22110B | IHC-P,ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-NOS3 (eNOS)
IHC staining of Purified anti-NOS3 (eNOS) (clone W22110B) on... 
EA.hy926 cells (positive cell line) were fixed with 4% PFA F... 
Whole cell extracts (15 µg total protein) from indicated cel... 
EA.hy926 cells (positive control, filled histogram) or HeLa ... -
Alexa Fluor® 488 anti-NOS3 (eNOS)
EA.hy926 cells (positive cell line, filled histogram) and He... -
Alexa Fluor® 647 anti-NOS3 (eNOS)
IHC staining with Alexa Fluor® 647 anti-NOS3 (eNOS) (clone W... 
EA.hy926 cells (positive cell line, filled histogram) and He...
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