- Clone
- Tau 5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Microtubule-associated protein tau, PHF-tau, paired helical filament-tau, neurofibrillary tangle, microtubule-associated protein tau, isoform 4, G protein beta1/gamma2 subunit-interacting factor 1, DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND
- Isotype
- Mouse IgG1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
806409 | 25 µg | 128€ | ||||
806410 | 100 µg | 296€ |
Tau proteins are microtubule-associated protein (MAPs) which are abundant in neurons of the central nervous system, but are also expressed at very low levels in CNS astrocytes and oligodendrocytes and elsewhere. One of tau's main functions is to modulate the stability of axonal microtubules. Tau is active primarily in the distal portions of axons providing microtubule stabilization as well as flexibility. Pathologies and dementias of the nervous system such as Alzheimer's disease feature tau proteins that have become defective and no longer stabilize microtubules properly. As a result, tau forms aggregates with specific structural properties referred to as Paired Helical Filaments (PHFs) that are a characteristic of many different types of dementias, known as tauopathies.
Tau has two primary ways of controlling microtubule stability: isoforms and phosphorylation. Six tau isoforms exist in human brain tissue, and they are distinguished by the number of binding domains. Three isoforms have three binding domains and the remaining three have four binding domains. The binding domains are located in the carboxy-terminus of the protein and are positively-charged (for binding to the negatively-charged microtubule). Tau isoforms with four binding domains are better at stabilizing microtubules than those with three binding domains.
Thus, in the human brain, the tau proteins constitute a family of six isoforms with the range from 352-441 amino acids. They also differ in either zero, one or two inserts of 29 amino acids at the N-terminal part (exon 2 and 3), and three or four repeat-binding regions at the C-terminus. So, the longest isoform in the CNS has four repeats (R1, R2, R3 and R4) and two inserts (441 amino acids total), while the shortest isoform has three repeats (R1, R3 and R4) and no insert (352 amino acids total). Tau is also a phosphoprotein with 79 potential Serine (Ser) and Threonine (Thr) phosphorylation sites on the longest tau isoform. Phosphorylation has been reported on approximately 30 of these sites in normal tau proteins. Mechanisms that drive tau lesion formation in the highly prevalent sporadic form of AD are not fully understood, but appear to involve abnormal post-translational modifications (PTMs) that influence tau function, stability, and aggregation propensity.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
This antibody is specific for an epitope that lies between amino acids 210-230 of human Tau.
-
Application References
(PubMed link indicates BioLegend citation) -
- Lasagna-Reeves CA, et al. 2012. FASEB J. 26:1946. (WB, IHC-P) Pubmed
- Horowitz PM, et al. 2004. J Neurosci. 24:7895. (WB)
- RRID
-
AB_2814543 (BioLegend Cat. No. 806409)
AB_2814543 (BioLegend Cat. No. 806410)
Antigen Details
- Structure
- Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
- Distribution
-
Tissue distribution: Central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: Cytoskeleton, nucleus, plasma membrane, and cytosol. - Function
- Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
- Interaction
- Tau interacts with: Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, and alpha-synuclein.
- Cell Type
- Neurons
- Biology Area
- Cell Biology, Cell Proliferation and Viability, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Synaptic Biology
- Molecular Family
- Tau
- Gene ID
- 4137 View all products for this Gene ID
- UniProt
- View information about Tau 210-230 on UniProt.org
Related FAQs
Other Formats
View All Tau, 210-230 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Tau, 210-230 | Tau 5 | WB,IHC-P |
HRP anti-Tau, 210-230 | Tau 5 | WB,IHC-P |
Alexa Fluor® 594 anti-Tau, 210-230 | Tau 5 | IHC-P |
Biotin anti-Tau, 210-230 | Tau 5 | IHC-P |
Alexa Fluor® 488 anti-Tau, 210-230 | Tau 5 | IHC-P |
Alexa Fluor® 647 anti-Tau, 210-230 | Tau 5 | IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Tau, 210-230
Western blot of purified anti-Tau, 210-230 antibody (clone T... IHC staining of purified anti-Tau, 210-230 antibody (clone T... IHC staining of purified anti-Tau, 210-230 antibody (clone T... IHC staining of purified anti-Tau, 210-230 antibody (clone T... -
HRP anti-Tau, 210-230
Western blot of HRP anti-Tau, 210-230 antibody (clone Tau 5)... IHC staining of HRP anti-Tau, 210-230 antibody (clone Tau 5)... IHC staining of HRP anti-Tau, 210-230 antibody (clone Tau 5)... -
Alexa Fluor® 594 anti-Tau, 210-230
IHC staining of Alexa Fluor® 594 anti-Tau, 210-230 antibody ... IHC staining of Alexa Fluor® 594 anti-Tau, 210-230 antibody ... -
Biotin anti-Tau, 210-230
IHC staining Biotin anti-Tau, 210-230 antibody (clone Tau 5)... IHC staining Biotin anti-Tau, 210-230 antibody (clone Tau 5)... -
Alexa Fluor® 488 anti-Tau, 210-230
IHC staining of Alexa Fluor® 488 anti-Tau, 210-230 antibody ... IHC staining of Alexa Fluor® 488 anti-Tau, 210-230 antibody ... -
Alexa Fluor® 647 anti-Tau, 210-230
IHC staining of Alexa Fluor® 647 anti-Tau, 210-230 antibody ... IHC staining of Alexa Fluor® 647 anti-Tau, 210-230 antibody ... IHC staining of Alexa Fluor® 647 anti-Tau, 210-230 antibody ...
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