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Alexa Fluor® 594 anti-c-Myc Antibody

Pricing & Availability
Clone
9E10 (See other available formats)
Regulatory Status
RUO
Other Names
myc tag, c-Myc tag, cMyc tag, Myc epitope tag
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
9E10_A594_c-Myc_Antibody_1_110317
HeLa cells transiently transfected with c-Myc tag fused protein were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:1000 diluted (0.5 µg/ml) Alexa Fluor® 594 anti- c-Myc antibody (red). Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective. For negative control and titration data, refer to second image.
  • 9E10_A594_c-Myc_Antibody_1_110317
    HeLa cells transiently transfected with c-Myc tag fused protein were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:1000 diluted (0.5 µg/ml) Alexa Fluor® 594 anti- c-Myc antibody (red). Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective. For negative control and titration data, refer to second image.
  • 9E10_A594_c-Myc_Antibody_2_110317
    HeLa cells transiently transfected with c-Myc tag fused protein or non-transfected were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:250 (2 µlg/ml), 1: 1000 (0.5 µg/ml) and 1:2500 diluted (0.2 µg/ml) Alexa Fluor® 594 anti- c-Myc antibody (red). Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective using KEYENCE BZ-X700 fluorescence microscope
Compare all formats See Alexa Fluor® 594 spectral data
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626805 25 µg 128€
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Description

The 9E10 monoclonal antibody recognizes the amino acid sequence EQKLISEEDL, which is a specific portion of the human c-myc gene product. This antibody is highly specific and is unlikely to alter the activity of the cloned sequence.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Amino acids 408-439, C-terminal region of human c-myc
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.2 - 2.0 μg/ml (1:250 - 1:2500 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Application Notes

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Rao H, et al. 2001. Nature. 410:955.
  2. Peters CS, et al. 2001. J Biol Chem. 276:13718.
  3. Munro S, Pelham HR. 1987. Cell. 48:899.
  4. Evan GI, et al. 1985. Mol Cell Biol. 5:3610.
Product Citations
  1. Wang J, et al. 2022. Front Immunol. 13:863346. PubMed
RRID
AB_2721374 (BioLegend Cat. No. 626805)

Antigen Details

Biology Area
Cell Biology, Protein Purification, Protein Synthesis
Antigen References
  1. Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982.
  2. Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217.
  3. Battey J, et al. 1983. Cell 34:779.
  4. Beimling P, et al. 1985. Biochemistry 24:6349.
Gene ID
4609 View all products for this Gene ID
UniProt
View information about c-Myc on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 10.03.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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