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Alexa Fluor® 594 anti-mouse/human PNAd Antibody

Pricing & Availability
Clone
MECA-79 (See other available formats)
Regulatory Status
RUO
Other Names
PNAd, Peripheral Node Addressin, MECA-79
Isotype
Rat IgM, κ
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Product Citations
publications
MECA-79_A594_PNAd_Antibody_1_070620
Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 594 anti-mouse/human PNAd (clone MECA-79) antibody (red) and Alexa Fluor® 647 anti-human CD8a (clone C8/144B) antibody (green) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
  • MECA-79_A594_PNAd_Antibody_1_070620
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 594 anti-mouse/human PNAd (clone MECA-79) antibody (red) and Alexa Fluor® 647 anti-human CD8a (clone C8/144B) antibody (green) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
  • MECA-79_A594_PNAd_Antibody_2_070620
    C57BL/6 mouse frozen lymph node section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL PNAd (clone MECA-79) Alexa Fluor® 594 (red), B220 (clone RA3-6B2) Brilliant Violet 421™ (blue), and CD3 (clone 17A2) Alexa Fluor® 647 (green), overnight at 4°C. The image was captured by 10X objective.
Compare all formats See Alexa Fluor® 594 spectral data
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120805 100 µg 306€
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Description

MECA-79 recognizes a carbohydrate epitope shared with a group of sulfation decorated sialomucins, including sulfated ligands for CD62L (CD34, GlyCAM-1, Sgp200, and a subset of MAdCAM-1). This set antigens has been referred to as peripheral node addressin (PNAd) with the molecular mass 50-250 kD. It has been identified that GlcNAc-6-SO4 sulfation contributes to MECA-79 binding and the core 1 β1,3-N-acetylglucosaminyltransferase is required for the generation of the MECA-79 epitope. MECA-79 is expressed on high endothelial venules (HEV) of lymphoid tissues, chronic inflamed tissues and rheumatoid synovia. The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues. MECA-79 antibody reacts with mouse, human and many other species PNAd and blocks L-selectin-dependent lymphocyte adhesion in vitro and in vivo.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse lymph node stromal cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2.5 - 10 µg/mL is suggested. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking1-4 of cell adhesion, immunohistochemical staining1,2,6 of acetone-fixed frozen sections and 4% paraformaldehyde-fixed paraffin-embedded sections, flow cytometry10,11, immunoprecipitation3,5, and Western blotting6,11.

Application References

(PubMed link indicates BioLegend citation)
  1. Streeter PR, et al. 1988. J. Cell Biol. 107:1853. (IHC, Block)
  2. Michie SA, et al. 1993. Amer. J. Path. 143:1688. (IHC, Block)
  3. Berg EL, et al. 1991. J. Cell Biol. 114:343. (IP, Block)
  4. Berg EL, et al. 1993. Nature 366:695. (Block)
  5. Hemmerich S, et al. 1994. J. Exp. Med. 180:2219. (IP)
  6. Samulowitz U, et al. 2002. Am. J. Path. 160:1669. (IHC, WB)
  7. Burke SD, et al. 2007. Diabetes 56:2919. PubMed
  8. Hirakawa J, et al. 2010. J. Biol. Chem. 285:40864. PubMed
  9. Thomas SN, et al. 2012. J. Immunol. 189:2181. PubMed.
  10. Izawa D, et al. 1999. Int. Immunol. 11(12):1989-98. (FC, ICC)
  11. Sinha RK, et al. 2006. Immunology. 119(4):461-9. (FC, IHC-F, WB)
Product Citations
  1. El Shikh MEM, et al. 2022. Front Med (Lausanne). 9:1013660. PubMed
  2. Kamioka Y, et al. 2023. Cell Rep. 112580:42. PubMed
RRID
AB_2650843 (BioLegend Cat. No. 120805)

Antigen Details

Structure
Sulphated CD34, GlyCAM-1 and MAdCAM-1, Sgp200, sialomucin
Distribution

High endothelial venules (HEV) in lymphoid tissues, inflamed tissues and rheumatoid synovia

Function
Leukocyte homing, rolling
Ligand/Receptor
CD62L
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules
Antigen References

1. Streeter PR, et al. 1988. J. Cell Biol. 107:1853.
2. Berg EL, et al. 1993. Nature 366:695.
3. Hemmerich S, et al. 1994. J. Exp. Med. 180:2219.
4. Mitoma J, et al. 2003. J. Biol. Chem. 278:9953.
5. Bruehl RE, et al. 2000. J. Biol. Chem. 275:32642.
6. Bistrup A, et al. 2004. Am. J. Pathol. 164:1635

Gene ID
123803 View all products for this Gene ID 18203 View all products for this Gene ID
UniProt
View information about PNAd on UniProt.org

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Go To Top Version: 3    Revision Date: 07.06.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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