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Alexa Fluor® 647 anti-CREB1 Phospho (Ser133) Antibody

Pricing & Availability
Clone
A22026B (See other available formats)
Regulatory Status
RUO
Other Names
CAMP responsive element binding protein 1; CREB-1; Cyclic Adenosine 3',5'-Monophosphate Response Element-Binding Protein; CAMP-Responsive Element-Binding Protein 1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A22026B_A647_CREB1_ICFC_070124
Serum starved Jurkat cells untreated (negative control, open histogram) or treated with Forskolin (positive control, filled histogram) were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-CREB1 Phospho (Ser133) (clone A22026B), or Alexa Fluor® 647 mouse IgG2b, κ isotype control (open histogram, dashed line) (Cat No. 400330).
  • A22026B_A647_CREB1_ICFC_070124
    Serum starved Jurkat cells untreated (negative control, open histogram) or treated with Forskolin (positive control, filled histogram) were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-CREB1 Phospho (Ser133) (clone A22026B), or Alexa Fluor® 647 mouse IgG2b, κ isotype control (open histogram, dashed line) (Cat No. 400330).
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632553 25 tests 144€
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632554 100 tests 380€
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Description

Cyclic AMP (cAMP)-response element-binding protein 1 (CREB1) is a phosphorylation-dependent transcription factor that binds to the cAMP response element (CRE) in response to certain stimuli. CREB1 contains a basic leucine zipper (bZIP) DNA-binding domain, 1 dimerization domain, and 9 serine residues in 1 kinase inducible domain phosphorylated by multiple kinases, including Ca2+/CaM-dependent kinase (CaMK) II and IV, protein kinase A (PKA), protein kinase C (PKC), mitogen/stress-activated kinase (MSK), ribosomal S6 kinase (RSK), AKT, and MAPKAP kinase 2 (MK2). Both the protein levels and activation of CREB1 are vital for maintaining cellular homeostasis in physiological and pathological conditions. Phosphorylation of CREB1 at Ser133 promotes the recruitment of co-activator proteins CBP and p300, which has been proposed to increase transcription of CREB-dependent genes. Ser133 can also be phosphorylated by other proteins such as MSK1/2 in response to the activation of MAPK signaling.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide for human CREB1 phosphorylated at serine 133
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Additional Product Notes

This antibody is not suitable to use in ICC and/or IHC.

RRID
AB_3662314 (BioLegend Cat. No. 632553)
AB_3662314 (BioLegend Cat. No. 632554)

Antigen Details

Structure
CREB1 contains 327 amino acids at a predicted molecular weight around 37 kD.
Distribution

Ubiquitously expressed/Nucleus

Function
Under physiological conditions, CREB1 maintains cellular survival, proliferation, anti-inflammatory immune responses and modulates the development of the nervous system by serving a neuroprotective role. Under pathological conditions, increased levels of CREB1 is associated with acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), Hodgkin’s lymphoma, chronic lymphatic leukemia (CLL), melanoma, hepatocellular, renal cell, ovarian, prostate, lung, gastric, esophageal, pancreatic, and breast tumors. CREB1 is also involved in the aging process by regulating the downstream signals of mitochondria dysfunction.
Interaction
CREB1 interacts with CREBBP, CRTC2, EP300, CRTC1, JUN, AKT1, CAMK4, FOS, RPS6KA1, RPS6KA3, CaMKII, CaMKIV, PKA, PKC, MSK, RSK, AKT, and MK2.
Cell Type
B cells, Endothelial cells, Fibroblasts, Granulocytes, Macrophages, NK cells, T cells
Biology Area
Cell Biology, Transcription Factors
Antigen References
  1. Steven A, et al. 2020. Cell Mol Life Sci. 77:4049-4067.
  2. Lin PH, et al. 2020. Diagnostics (Basel). 10:295.
  3. Sakamoto K, et al. 2011. J Neurochem. 116:1-9.
  4. Wen AY, et al. 2010. J Immunol. 185:6413-9.
  5. Arnould T, et al. 2002. EMBO J. 21:53-63.
  6. Naqvi S, et al. 2014. Biochem J. 458:469-79.
Gene ID
1385 View all products for this Gene ID
UniProt
View information about CREB1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07.01.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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