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Alexa Fluor® 647 anti-human CD63 Antibody

Pricing & Availability
Clone
H5C6 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
LIMP, LAMP-3, Melanoma-associated antigen (ME491), Pltgp40, gp55
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
H5C6_AF647_CD63_Antibody_FC_101712
Thrombin-activated human peripheral blood platelets were stained with CD63 (clone H5C6) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).
  • H5C6_AF647_CD63_Antibody_FC_101712
    Thrombin-activated human peripheral blood platelets were stained with CD63 (clone H5C6) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).
Compare all formats See Alexa Fluor® 647 spectral data
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353015 25 tests 100€
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353016 100 tests 254€
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Description

CD63 is a 53 kD type III lysosomal glycoprotein also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491). CD63 is a member of the tetraspan transmembrane superfamily (TM4SF) protein expressed on activated platelets, monocytes/macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle cells. CD63 may be involved in platelet activation and is thought to function as a transmembrane adaptor protein. CD63 has been shown to associate with CD9, CD81, VLA-3, and VLA-6.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
T cell line HPB-ALL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1, immunofluorescence2, and immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Hildreth JE, et al. 1991. Blood 77:121. (IP, WB)
  2. Beatty WL, et al. 2006. J. Cell Sci. 119:350. (IF)
Product Citations
  1. Lanna A, et al. 2022. Nat Cell Biol. 24:1461. PubMed
  2. Emmons TR, et al. 2021. Cancer Immunol Res. 9:790. PubMed
  3. Helmink BA, et al. 2020. Nature. 577:549. PubMed
  4. Ambrose AR, et al. 2020. Proc Natl Acad Sci U S A. 117:23717. PubMed
  5. Halder LD, et al. 2020. Nat Commun. 2.077083333. PubMed
RRID
AB_2561662 (BioLegend Cat. No. 353015)
AB_2561662 (BioLegend Cat. No. 353016)

Antigen Details

Structure
Tetraspan transmembrane superfamily (TM4SF), type III lysosomal glycoprotein, 53 kD
Distribution

Activated platelets, monocytes, macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle

Function
Platelet activation
Interaction
CD9, CD81, VLA-3, and VLA-6
Cell Type
B cells, Endothelial cells, Fibroblasts, Macrophages, Monocytes, Osteoclasts, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Azorsa DO, et al. 1991. Blood 78:280.
  2. Kishimoto T, et al. Eds. 1997. Leukocyte Typing V1. Oxford University Press New York.
  3. Hildreth JE, et al. 1991. Blood 77:121.
  4. Anzai N, et al. 2002. Blood 99:4413.
Gene ID
967 View all products for this Gene ID
UniProt
View information about CD63 on UniProt.org
Go To Top Version: 3    Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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