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Alexa Fluor® 647 anti-human HIF1α Antibody

Pricing & Availability
Clone
546-16 (See other available formats)
Regulatory Status
RUO
Other Names
MOP1, ARNT interacting protein, hypoxia inducible factor 1 alpha
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
546-16_AF647_HIF1a_Antibody_FC_032014.jpg
Hela cells were treated overnight (top) or untreated (bottom) with CoCl2, then intracellularly stained with HIF1α (clone 546-16) Alexa Fluor® 647 (filled histogram) or mouse IgG2b, κ Alexa Fluor® 647 isotype control (open histogram).
  • 546-16_AF647_HIF1a_Antibody_FC_032014.jpg
    Hela cells were treated overnight (top) or untreated (bottom) with CoCl2, then intracellularly stained with HIF1α (clone 546-16) Alexa Fluor® 647 (filled histogram) or mouse IgG2b, κ Alexa Fluor® 647 isotype control (open histogram).
  • 546-16_AF647_HIF1a_Antibody_FC_2_032014.jpg
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359705 25 tests 137€
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359706 100 tests 296€
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Description

HIF1, hypoxia induced factor 1, is a heterodimeric basic helix-loop-helix transcription factor composed of HIF1α and HIF1β. HIF1β is an aryl hydrocarbon receptor nuclear translocator (Arnt). Under normoxic conditions, HIF1α undergoes prolyl hydroxylation, which leads to degradation of HIF1α by the proteasome. Oxygen-starvation inhibits prolyl hydroxylation and prevents proteasomal degradation of HIF1α. Following nuclear translocation, HIF1α binds to the core DNA sequence 5'-[AG]CGTG-3' within the hypoxia responsive element (HRE); only the HIF1 heterodimer can bind DNA. HIF1 is a master regulator of the response to hypoxia and induces transcription of multiple genes including VEGF, glucose transporters, and proteins that aid in adaptation to hypoxic conditions. HIF1 protein is also involved in angiogenesis and energy metabolism.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant HIF1α
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Koers J, et al. 2023. Front Immunol. 13:1082154. PubMed
  2. Bhattacharya S, et al. 2020. Front Bioeng Biotechnol. 1.055555556. PubMed
  3. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  4. Dong Y, et al. 2020. J Leukoc Biol. 108:1711. PubMed
RRID
AB_2563349 (BioLegend Cat. No. 359705)
AB_2563349 (BioLegend Cat. No. 359706)

Antigen Details

Structure
92.6 kD, contains one basic helix-loop-helix structure (bHLH), one PAS associated C - terminal (PAC) domain and two PER-ARNT-SIM (PAS) domains
Distribution

Under normoxic conditions detected in the cytoplasm; oxygen starvation causes nuclear translocation

Function
Master regulator of hypoxia in the adaptive immune response; under low oxygen conditions, induces expression of multiple genes such as VEGF, erythropoietin, and other genes that aid in adaptation to low oxygen concentrations
Interaction
Heterodimer binds 5'-[AG]CGTG-3' sequence of DNA in the hypoxia response element of target genes; interacts with COPS5, EP300, CITED2
Biology Area
Cell Biology, Immunology, Innate Immunity, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Wang GL and Semenza GL. 1993. Proc. Natl. Acad. Sci. 90:4304.
2. Wang GL and Semenza GL. 1993. J. Biol. Chem. 268:21513.
3. Wang GL and Semenza GL. 1995. J. Biol. Chem. 270:1230.
4. Galbraith MD, et al. 2013. Cell. 153:1327.

Gene ID
3091 View all products for this Gene ID
UniProt
View information about HIF1alpha on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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