Alexa Fluor 647 anti-mouse Aiolos Antibody anti-Aiolos

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Clone: 8B2 (See other available formats)
Regulatory Status: RUO
Other Names: IKAROS family zinc finger 3 (Ikzf3), zinc finger protein Aiolos, Zfpn1a3, Znfn1a3
Isotype: Mouse IgG1, κ
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Product Citations: publications

8B2_AF647_Aiolos_Antibody_FC_1_022014 — C57BL/6 mouse splenocytes were stained with CD3 Alexa Fluor® 488, fixed and permeabilized with FOXP3 Fix/Perm Buffer Set, and then stained with Aiolos (clone 8B2) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).

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653205

25 µg

128€

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653206

100 µg

315€

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Description

Aiolos, an Ikaros family member, is also known as Izkf3. It has a molecular weight of 58 kD and shows 70% homology to the Ikaros isoform, Ik-1, particularly in the DNA binding and C-terminal domains. Aiolos can form a heteromer with Ikaros or exist as a homodimer. The homomeric complex is a potent transcriptional activator. Unlike Ikaros, Aiolos is expressed in late fetal thymus and adult lymphoid organs. Among the adult hematopoietic progenitors, Aiolos is expressed at low levels in mulitpotent but not in pluripotent stem cells. When these multipotent progenitors become more restricted to T and B lymphoid pathways there is an upregulation in Aiolos expression. The highest expression of Aiolos is detected in the spleen (especially in B-cells) followed by thymus and bone marrow, and cannot be detected in the non-lymphoid organs. Aiolos plays an important role in lymphocyte proliferation and differentiation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: N-terminal half of mouse Aiolos fused to GST
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: intracellular flow cytometry¹, immunoprecipitation¹, and Western blotting².

Does not cross-react with human.

View more applications data for a different format of this clone in our Scientific Poster Library.

This product is for research use only, cannot be used on or in humans, and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California.
RRID: AB_2563238 (BioLegend Cat. No. 653205)
AB_2563238 (BioLegend Cat. No. 653206)

Antigen Details

Structure: 509 amino acids with predicted MW of 58 kD
Distribution: Nucleus, cytoplasm
Restricted to lymphoid lineage, expressed in B, NK and T cells
Function: Transcription factor that plays an important role in the regulation of lymphocyte differentiation; plays an essential role in regulation of B-cell differentiation, proliferation, and maturation to an effector state
Interaction: Homodimer and heterodimer with other IKAROS family members; interacts with IKZF4, IKZF5, IKZF1, HRAS, FOXP3, and BCL21L isoform Bcl-X(L); associates with histone deacetylase complexes containing HDAC1, MTA2 and SIN3A
Cell Type: B cells, NK cells, T cells
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Innate Immunity, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Morgan B, et al. 1997. EMBO J. 16:2004.
2. Wang JH, et al. 1998. Immunity 9:543.
3. Cariappa A, et al. 2001. Immunity 14:603.
Gene ID: 22780 View all products for this Gene ID
UniProt: View information about Aiolos on UniProt.org

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Related Pages & Pathways

Pathways

CD4 and CD8 T-Cell Lineage

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Other Formats

View All Aiolos Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse Aiolos	8B2	WB,ICFC,IP
PE anti-mouse Aiolos	8B2	ICFC
Alexa Fluor® 647 anti-mouse Aiolos	8B2	ICFC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

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