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Alexa Fluor® 647 anti-mouse CD252 (OX40L) Antibody

Pricing & Availability
Clone
RM134L (See other available formats)
Regulatory Status
RUO
Other Names
OX-40L, CD134L, CD252, TNFSF4
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
RM134L_AF647_011812
C57BL/6 splenocytes were stimulated with anti-mouse IgM and CD40 antibodies for 3 days, then stained with OX40L (clone RM134L) Alexa Fluor® 647 (filled histogram) or rat IgG2b Alexa Fluor® 647 isotype control (open histogram).
  • RM134L_AF647_011812
    C57BL/6 splenocytes were stimulated with anti-mouse IgM and CD40 antibodies for 3 days, then stained with OX40L (clone RM134L) Alexa Fluor® 647 (filled histogram) or rat IgG2b Alexa Fluor® 647 isotype control (open histogram).
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
108809 25 µg 81€
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108810 100 µg 184€
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Description

CD252 is a 35 kD member of the TNF superfamily and is also known as OX40 ligand, OX40L, and CD134L. The CD252 is expressed on activated B cells and antigen presenting cells. CD252 interacts with OX40 antigen (CD134), expressed predominantly on activated T cells to increase proliferation and IL-2 production and to enhance proliferation and immunoglobulin secretion in activated B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Rat NRK-52E cell line transfected with mouse OX-40L
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The RM134L antibody can block the costimulatory activity of OX40L. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections3, and in vivo and in vitro blocking of OX-40L-OX-40 functional interaction1,2,4. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108808).

Application References

(PubMed link indicates BioLegend citation)
  1. Akiba H, et al. 1999. J. Immunol. 162:7058. (Block)
  2. Akiba H, et al. 2000. J. Exp. Med. 191:375. (Block)
  3. Hoshino A, et al. 2003. Eur. J. Immunol. 33:861. (IHC)
  4. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Block)
  5. Zheng X, et al. 2010. Invest Ophthalmol Vis Sci. 51:3076. PubMed
Product Citations
  1. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
  2. Elayeb R, et al. 2016. Haematologica. 101: 209 - 218. PubMed
RRID
AB_2207379 (BioLegend Cat. No. 108809)
AB_2207379 (BioLegend Cat. No. 108810)

Antigen Details

Structure
NGF/TNF superfamily, 35 kD
Distribution

Activated B cells, antigen presenting cells

Function
B-cell/T-cell interaction, costimulation
Ligand/Receptor
OX40 (CD134)
Cell Type
Antigen-presenting cells, B cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Akiba H, et al. 1999. J. Immunol. 162:7058.
2. Stüber E, et al. 1995. Immunity 2:507.
3. Baum PR, et al. 1994. EMBO J. 13:3992.

Gene ID
22164 View all products for this Gene ID
UniProt
View information about CD252 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 02.04.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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