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Alexa Fluor® 647 anti-mouse CD85k (gp49 Receptor) Antibody

Pricing & Availability
Clone
H1.1 (See other available formats)
Regulatory Status
RUO
Other Names
Leukocyte immunoglobulin-like receptor, subfamily B, member 4 (Lilrb4), gp49, gp49B1, gp49B, ILT3
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
H1.1_AF647_gp49B_Antibody_FC_1_051413
C57BL/6 mouse bone marrow cells were stained with CD11b PE and CD85k (clone H1.1) Alexa Fluor® 647 or Armenian hamster IgG Alexa Fluor® 647 isotype control (bottom).
  • H1.1_AF647_gp49B_Antibody_FC_1_051413
    C57BL/6 mouse bone marrow cells were stained with CD11b PE and CD85k (clone H1.1) Alexa Fluor® 647 or Armenian hamster IgG Alexa Fluor® 647 isotype control (bottom).
  • H1.1_AF647_gp49B_Antibody_FC_2_051413
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
144905 25 µg 118€
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144906 100 µg 287€
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Description

gp49, also known as ILT3, gp49b1 or CD85k, is a 49 kD glycoprotein expressed on activated NK cells and myeloid cells. It structurally resembles killer inhibitory receptors (KIR). gp49B is a type I membrane protein belonging to the Ig superfamily and contains an immunoreceptor tyrosine-based inhibitory motif (ITIM), while gp49A is a truncated molecule without ITIM. Upon activation following interaction with integrin avb3, the cytoplasmic tail associates with SHP-1 and SHP-2, which results in downregulation of cell activation and cytokine production.  

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
gp49B-Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone H1.1 recognizes both gp49A and gp49B. Additional reported applications (for the relevant formats) include in vitro activation of gp49B to inhibit cytokine production.  The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team).

Application References

(PubMed link indicates BioLegend citation)
  1. Wang LL, et al. 2000. J. Immunol. 164:5215. (Activ)
Product Citations
  1. He D, et al. 2022. Immunity. 55:159. PubMed
  2. Kretzschmar F, et al. 2021. Biology (Basel). 10:. PubMed
  3. Plenter RJ, et al. 2018. Cell Immunol. 332:101. PubMed
  4. Su R, et al. 2020. Cancer Cell. 38(1):79-96.e11. PubMed
  5. Van Gool F, et al. 2019. Immunity. 50:362. PubMed
  6. Lee HN, et al. 2022. JCI Insight. 7:. PubMed
RRID
AB_2562043 (BioLegend Cat. No. 144905)
AB_2562043 (BioLegend Cat. No. 144906)

Antigen Details

Structure
Type I membrane protein belonging to Ig superfamily
Distribution
Myeloid cells, activated NK cells
Function
Inhibitory activity mediated by ITIM motif
Interaction
SHP-1 and SHP-2
Ligand/Receptor
Integrin αvβ3, collagen
Cell Type
NK cells, Dendritic cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Castells, MC, et al. 1994. J. Biol. Chem. 269:8393.
2. Katz HR, et al. 1996. P. Natl. Acad. Sci. USA 93:10809.
3. Kuroiwa A, et al. 1998. J. Biol. Chem. 273:1070.
4. Wang LL, et al. 2000. J. Immunol. 164:5215.
5. Jiang L, et al. 2009. Eur. J. Immunol. 39:1167.

Gene ID
14728 View all products for this Gene ID
UniProt
View information about CD85k on UniProt.org
Go To Top Version: 2    Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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