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Alexa Fluor® 647 anti-mouse H-2Kb Antibody

Pricing & Availability
Clone
AF6-88.5 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class I
Isotype
Mouse (BALB/c) IgG2a, κ
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Product Citations
publications
AF6-88dot5_ALX647_042706
C57BL/6 mouse splenocytes stained with AF6-88.5 Alexa Fluor® 647
  • AF6-88dot5_ALX647_042706
    C57BL/6 mouse splenocytes stained with AF6-88.5 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
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116511 25 µg 81€
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116512 100 µg 184€
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Description

The AF6-88.5 antibody reacts with H-2Kb MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kb haplotype. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C57BL mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunohistochemical staining of acetone-fixed frozen sections3. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections.

Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v).

Application References

(PubMed link indicates BioLegend citation)
  1. Loken MR, et al. 1982. J. Immunol. Methods 50:R85.
  2. Wall KA, et al. 1983. J. Immunol. 131:1056.
  3. Andersson M, et al. 1998. J. Immunol. 161:6475.
  4. Shao H, et al. 2005. J. Immunol. 175:1851.
  5. Hui S, et al. 2005. J. Immunol. 175:1851. PubMed
  6. Zhou K, et al. 2010. Cytotherapy. 12:735. PubMed
  7. Desvignes L, et al. 2012. J Immunol. 188:6205. PubMed
Product Citations
  1. Sparbier CE, et al. 2023. Nat Cell Biol. 25:258. PubMed
  2. Falahat R, et al. 2023. Nat Commun. 14:1573. PubMed
  3. del Rio ML, et al. 2022. Front Immunol. 13:887348. PubMed
  4. Mugarza E, et al. 2022. Sci Adv. 8:eabm8780. PubMed
  5. Capietto AH, et al. 2020. J Exp Med. 217:00:00. PubMed
  6. Jensen U, et al. 2008. J Cell Sci. 121:609. PubMed
  7. del Rio ML, et al. 2021. Transl Res. Online ahead of print.. PubMed
  8. Marangoni F, et al. 2021. Cell. . PubMed
  9. Hubbard JJ, et al. 2020. J Exp Med. :217. PubMed
  10. Glassman CR, et al. 2021. Cell. 184(4):983-999.e24. PubMed
  11. Rio M, et al. 2012. J Immunol. 188:4885. PubMed
  12. Song S, et al. 2021. Front Immunol. 12:705140. PubMed
  13. Zhang Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  14. Martin-Granados C, et al. 2015. J Mol Cell Biol. . PubMed
  15. Huanga J, et al. 2013. J Immunol Methods. 387:254. PubMed
RRID
AB_492917 (BioLegend Cat. No. 116511)
AB_492917 (BioLegend Cat. No. 116512)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Kb haplotype

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Review Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.

Gene ID
14972 View all products for this Gene ID
UniProt
View information about H-2Kb on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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